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iFluor® 350-dUTP *1 mM in Tris Buffer (pH 7.5)*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Solvent | Water |
Spectral properties
| Correction Factor (260 nm) | 0.83 |
| Correction Factor (280 nm) | 0.23 |
| Extinction coefficient (cm -1 M -1) | 200001 |
| Excitation (nm) | 345 |
| Emission (nm) | 450 |
| Quantum yield | 0.951 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
Correction Factor (260 nm) 0.83 | Correction Factor (280 nm) 0.23 | Extinction coefficient (cm -1 M -1) 200001 | Excitation (nm) 345 | Emission (nm) 450 | Quantum yield 0.951 |
Dye-modified deoxyuridine 5'-triphosphates are widely used for the production of dye-labeled DNA via conventional enzymatic incorporation techniques, including reverse transcription, nick translation, random primed labeling, and PCR. These enzymatic fluorescence labeling methods are commonly employed in both fluorescence in situ hybridization (FISH) probes and microarray-based experiments. The iFluor® 350-dUTP conjugate, which emits blue fluorescence, is compatible with the SpectrumBlue™ filter set (SpectrumBlue™ is a trademark of Vysis). Compared to commonly used blue probes such as AMCA-6-dUTP, iFluor® 350 provides a significantly brighter signal, greater photostability, and is less affected by pH variations.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.83 |
| Correction Factor (280 nm) | 0.23 |
| Extinction coefficient (cm -1 M -1) | 200001 |
| Excitation (nm) | 345 |
| Emission (nm) | 450 |
| Quantum yield | 0.951 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| iFluor® 350 maleimide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
| iFluor® 350 amine | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
| iFluor® 350 hydrazide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
| iFluor® 350 Styramide *Superior Replacement for Alexa Fluor 350 tyramide* | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
| iFluor® 350 Tyramide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
| iFluor® 440-dUTP *1 mM in TE Buffer (pH 7.5)* | 434 | 480 | 400001 | 0.671 | 0.352 | 0.229 |
| iFluor®488-dUTP *1 mM in TE Buffer (pH 7.5)* | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
| iFluor® 560-dUTP *1 mM in TE Buffer (pH 7.5)* | 560 | 571 | 1200001 | 0.571 | 0.0482 | 0.069 |
Images
Figure 1. iFluor 350-dUTP
References
View all 50 references: Citation Explorer
Lanthanide Complex for Single-Molecule Fluorescent in Situ Hybridization and Background-Free Imaging.
Authors: Su, Fei and Chen, Shiyu and Liu, Yuanhua and Zhou, Jiajia and Du, Zhongbo and Luo, Xiongjian and Wen, Shihui and Jin, Dayong
Journal: Analytical chemistry (2024): 4430-4436
Authors: Su, Fei and Chen, Shiyu and Liu, Yuanhua and Zhou, Jiajia and Du, Zhongbo and Luo, Xiongjian and Wen, Shihui and Jin, Dayong
Journal: Analytical chemistry (2024): 4430-4436
CircHIPK3 promotes bone microvascular endothelial cell proliferation, migration and angiogenesis by targeting miR-7 and KLF4/VEGF signaling in steroid-induced osteonecrosis of the femoral head.
Authors: Peng, Peng and He, Wei and Zhang, Yi-Xi and Liu, Xiao-Hua and Chen, Zhen-Qiu and Mao, Ji-Gang
Journal: Advances in clinical and experimental medicine : official organ Wroclaw Medical University (2023): 43-55
Authors: Peng, Peng and He, Wei and Zhang, Yi-Xi and Liu, Xiao-Hua and Chen, Zhen-Qiu and Mao, Ji-Gang
Journal: Advances in clinical and experimental medicine : official organ Wroclaw Medical University (2023): 43-55
The Long Noncoding RNA Gm9866/Nuclear Factor-κB Axis Promotes Macrophage Polarization.
Authors: Liao, Xiaomin and Ruan, Xianxian and Chen, Xiubing and Zheng, Biaolin and Wu, Xianbin and Deng, Zhejun and Yang, Zhe and Wang, Feng and Qin, Shanyu and Jiang, Haixing
Journal: Mediators of inflammation (2023): 9991916
Authors: Liao, Xiaomin and Ruan, Xianxian and Chen, Xiubing and Zheng, Biaolin and Wu, Xianbin and Deng, Zhejun and Yang, Zhe and Wang, Feng and Qin, Shanyu and Jiang, Haixing
Journal: Mediators of inflammation (2023): 9991916
Inhibition of PLA2G4E/cPLA2 promotes survival of random skin flaps by alleviating Lysosomal membrane permeabilization-Induced necroptosis.
Authors: Lou, Junsheng and Wang, Xiangyang and Zhang, Haojie and Yu, Gaoxiang and Ding, Jian and Zhu, Xuwei and Li, Yao and Wu, Yaosen and Xu, Hui and Xu, Huazi and Gao, Weiyang and Xiao, Jian and Zhou, Kailiang
Journal: Autophagy (2022): 1841-1863
Authors: Lou, Junsheng and Wang, Xiangyang and Zhang, Haojie and Yu, Gaoxiang and Ding, Jian and Zhu, Xuwei and Li, Yao and Wu, Yaosen and Xu, Hui and Xu, Huazi and Gao, Weiyang and Xiao, Jian and Zhou, Kailiang
Journal: Autophagy (2022): 1841-1863
LINC01234 Accelerates the Progression of Breast Cancer via the miR-525-5p/Cold Shock Domain-Containing E1 Axis.
Authors: Yu, Jia and Zhang, Xinxin and He, Rongfang and Yang, Xiaoyan and Hu, Juan and Tan, Fang and Yao, Jinyu and Lei, Xiaoyong and Wen, Gebo
Journal: Disease markers (2022): 6899777
Authors: Yu, Jia and Zhang, Xinxin and He, Rongfang and Yang, Xiaoyan and Hu, Juan and Tan, Fang and Yao, Jinyu and Lei, Xiaoyong and Wen, Gebo
Journal: Disease markers (2022): 6899777
P38α deficiency in macrophages ameliorates murine experimental colitis by regulating inflammation and immune process.
Authors: Chen, Wei and Liang, Rui and Yi, Youcai and Zhu, Jinshui and Zhang, Jing
Journal: Pathology, research and practice (2022): 153881
Authors: Chen, Wei and Liang, Rui and Yi, Youcai and Zhu, Jinshui and Zhang, Jing
Journal: Pathology, research and practice (2022): 153881
Clostridium butyricum inhibits the progression of colorectal cancer and alleviates intestinal inflammation via the myeloid differentiation factor 88 (MyD88)-nuclear factor-kappa B (NF-κB) signaling pathway.
Authors: Zhou, Mingyao and Yuan, Wei and Yang, Bing and Pei, Wei and Ma, Jie and Feng, Qiang
Journal: Annals of translational medicine (2022): 478
Authors: Zhou, Mingyao and Yuan, Wei and Yang, Bing and Pei, Wei and Ma, Jie and Feng, Qiang
Journal: Annals of translational medicine (2022): 478
MACS-annexin V cell sorting of semen samples with high TUNEL values decreases the concentration of cells with abnormal chromosomal content: a pilot study.
Authors: El Fekih, Sahar and Gueganic, Nadia and Tous, Corinne and Ali, Habib Ben and Ajina, Mounir and Douet-Guilbert, Nathalie and Drapier, Hortense and Beauvillard, Damien and Morel, Frédéric and Perrin, Aurore
Journal: Asian journal of andrology (2022): 445-450
Authors: El Fekih, Sahar and Gueganic, Nadia and Tous, Corinne and Ali, Habib Ben and Ajina, Mounir and Douet-Guilbert, Nathalie and Drapier, Hortense and Beauvillard, Damien and Morel, Frédéric and Perrin, Aurore
Journal: Asian journal of andrology (2022): 445-450
LncRNA MINCR attenuates osteoarthritis progression via sponging miR-146a-5p to promote BMPR2 expression.
Authors: Li, Dongyun and Wang, Xiaoying and Yi, Tengda and Zhang, Lin and Feng, Lirui and Zhang, Mingxing and He, Yongsheng and Gang, Shunkui
Journal: Cell cycle (Georgetown, Tex.) (2022): 2417-2432
Authors: Li, Dongyun and Wang, Xiaoying and Yi, Tengda and Zhang, Lin and Feng, Lirui and Zhang, Mingxing and He, Yongsheng and Gang, Shunkui
Journal: Cell cycle (Georgetown, Tex.) (2022): 2417-2432
Oxidative Stress Is Associated with Telomere Interaction Impairment and Chromatin Condensation Defects in Spermatozoa of Infertile Males.
Authors: Berby, Benoit and Bichara, Cynthia and Rives-Feraille, Aurélie and Jumeau, Fanny and Pizio, Pierre Di and Sétif, Véronique and Sibert, Louis and Dumont, Ludovic and Rondanino, Chistine and Rives, Nathalie
Journal: Antioxidants (Basel, Switzerland) (2021)
Authors: Berby, Benoit and Bichara, Cynthia and Rives-Feraille, Aurélie and Jumeau, Fanny and Pizio, Pierre Di and Sétif, Véronique and Sibert, Louis and Dumont, Ludovic and Rondanino, Chistine and Rives, Nathalie
Journal: Antioxidants (Basel, Switzerland) (2021)