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iFluor® 488-streptavidin conjugate

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.This iFluor® 488-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor® 488 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. iFluor® 488-streptavidin conjugates has fluorescence excitation and emission maxima of ~491 nm and ~514 nm respectively. These spectral characteristics make it an excellent alternative to Alexa Fluor® 488-streptavidin conjugate (Alexa Fluor® is the trademark of Invitrogen). It is a very valuable tool for biotin-streptavidin-based biological assays and tests.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350-streptavidin conjugate3454502000010.9510.830.23
iFluor® 405-streptavidin conjugate4034273700010.9110.480.77
iFluor® 514-streptavidin conjugate5115277500010.8310.2650.116
iFluor® 532-streptavidin conjugate5375609000010.6810.260.16
iFluor® 555-streptavidin conjugate55757010000010.6410.230.14
iFluor® 594-streptavidin conjugate58760320000010.5310.050.04
iFluor® 633-streptavidin conjugate64065425000010.2910.0620.044
iFluor® 647-streptavidin conjugate65667025000010.2510.030.03
iFluor® 680-streptavidin conjugate68470122000010.2310.0970.094
iFluor® 700-streptavidin conjugate69071322000010.2310.090.04
iFluor® 750-streptavidin conjugate75777927500010.1210.0440.039
iFluor® 546-streptavidin conjugate54155710000010.6710.250.15
iFluor® 568-streptavidin conjugate56858710000010.5710.340.15
iFluor® 790-streptavidin conjugate78781225000010.1310.10.09
iFluor® 800-streptavidin conjugate80182025000010.1110.030.08
iFluor® 820-streptavidin conjugate82285025000010.110.16
iFluor® 840-streptavidin conjugate8368792000001-0.20.09
iFluor® 860-streptavidin conjugate85387825000010.10.14
Phalloidin-iFluor® 488 Conjugate4915167500010.910.210.11
Show More (10)

Citations

View all 5 citations: Citation Explorer
Isolation, characterization, and structure-based engineering of a neutralizing nanobody against SARS-CoV-2
Authors: Li, Tingting and Zhou, Bingjie and Li, Yaning and Huang, Suqiong and Luo, Zhipu and Zhou, Yuanze and Lai, Yanling and Gautam, Anupriya and Bourgeau, Salome and Wang, Shurui and others,
Journal: International Journal of Biological Macromolecules (2022): 1379--1388
A synthetic nanobody targeting RBD protects hamsters from SARS-CoV-2 infection
Authors: Li, Tingting and Cai, Hongmin and Yao, Hebang and Zhou, Bingjie and Zhang, Ning and van Vlissingen, Martje Fentener and Kuiken, Thijs and Han, Wenyu and GeurtsvanKessel, Corine H and Gong, Yuhuan and others,
Journal: Nature communications (2021): 1--13
A potent synthetic nanobody targets RBD and protects mice from SARS-CoV-2 infection
Authors: Li, Tingting and Cai, Hongmin and Yao, Hebang and Zhou, Bingjie and Zhang, Ning and Gong, Yuhuan and Zhao, Yapei and Shen, Quan and Qin, Wenming and Hutter, Cedric AJ and others,
Journal: BioRxiv (2020)
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996
New antidepressant mechanism of Yueju Pill: increasing ghrelin level by inhibiting gastric mTOR/S6K signaling pathway and sensitizing hippocampal GHS-R.
Authors: Zhang, Zhentao and Su, Dan and Lai, Meixizi and Song, Yonggui and Li, Huizhen and Yang, Ming and Zhu, Genhua and Liu, Hong and Ai, Zhifu
Journal: Heliyon

References

View all 47 references: Citation Explorer
A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647
Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50
Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label
Authors: Sun M, Du L, Gao S, Bao Y, Wang S.
Journal: Steroids (2010): 400
Page updated on October 28, 2024

Ordering information

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Unit size
200 ug
1 mg
Catalog Number
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Physical properties

Molecular weight

~52000

Solvent

Water

Spectral properties

Correction Factor (260 nm)

0.21

Correction Factor (280 nm)

0.11

Extinction coefficient (cm -1 M -1)

750001

Excitation (nm)

491

Emission (nm)

516

Quantum yield

0.91

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by AAT's iFluorTM 488-streptavidin conjugate (Green, Left) or streptavidin conjugated with Alexa Fluor&reg; 488  (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by AAT's iFluorTM 488-streptavidin conjugate (Green, Left) or streptavidin conjugated with Alexa Fluor&reg; 488  (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by AAT's iFluorTM 488-streptavidin conjugate (Green, Left) or streptavidin conjugated with Alexa Fluor&reg; 488  (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).