iFluor® 594 Tetrazine

Name: iFluor® 594 Tetrazine
Brand: AAT Bioquest
SKU: 1018
Price: 216 USD
Availability: InStock

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 594 tetrazine can be readily used to label TCO-modified biological molecules for fluorescence imaging and other fluorescence-based biological applications. The conjugates prepared with iFluor® 594 dye have spectral properties almost identical to the popular Cy3.5 and Alexa Fluor® 594. In most cases, antibody conjugates prepared with iFluor® 594 tend to have lower background than the spectrally similar dye conjugates, such as Cy3.5 and Alexa Fluor® 594 (Alexa Fluor® is the trademark of Invitrogen).

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 594 maleimide	587	603	200000¹	0.53¹	0.05	0.04
iFluor® 594 Styramide Superior Replacement for Alexa Fluor 594 tyramide	587	603	200000¹	0.53¹	0.05	0.04
iFluor® 594 Tyramide	587	603	200000¹	0.53¹	0.05	0.04
iFluor® 594 TCO	587	603	200000¹	0.53¹	0.05	0.04
iFluor® 488 Tetrazine	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 Tetrazine	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 647 Tetrazine	656	670	250000¹	0.25¹	0.03	0.03
ATTO 594 Tetrazine	602	621	120000	0.85	0.26	0.51

References

View all 50 references: Citation Explorer

Facile Click-Mediated Cell Imaging Strategy of Liposomal Azido Mannosamine Lipids via Metabolic or Nonmetabolic Glycoengineering.
Authors: Shen, Li and Cai, Kaimin and Yu, Jin and Cheng, Jianjun
Journal: ACS omega (2020): 14111-14115

Bioorthogonal Labeling Reveals Different Expression of Glycans in Mouse Hippocampal Neuron Cultures during Their Development.
Authors: Soares da Costa, Diana and Sousa, João C and Dá Mesquita, Sandro and Petkova-Yankova, Nevena I and Marques, Fernanda and Reis, Rui L and Sousa, Nuno and Pashkuleva, Iva
Journal: Molecules (Basel, Switzerland) (2020)

Surface-modified nanoerythrosomes for potential optical imaging diagnostics.
Authors: Fornasier, Marco and Porcheddu, Andrea and Casu, Anna and Raghavan, Srinivasa R and Jönsson, Peter and Schillén, Karin and Murgia, Sergio
Journal: Journal of colloid and interface science (2020): 246-253

Novel Liposomal Azido Mannosamine Lipids on Metabolic Cell Labeling and Imaging via Cu-Free Click Chemistry.
Authors: Shen, Li and Cai, Kaimin and Yu, Jin and Cheng, Jianjun
Journal: Bioconjugate chemistry (2019): 2317-2322

One-Pot Synthesis of Triazolobenzodiazepines Through Decarboxylative [3 + 2] Cycloaddition of Nonstabilized Azomethine Ylides and Cu-Free Click Reactions.
Authors: Ma, Xiaoming and Zhang, Xiaofeng and Qiu, Weiqi and Zhang, Wensheng and Wan, Bruce and Evans, Jason and Zhang, Wei
Journal: Molecules (Basel, Switzerland) (2019)

Page updated on December 3, 2024

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Catalog Number	1018
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Physical properties

Molecular weight	1158.21
Solvent	DMSO

Spectral properties

Absorbance (nm)	587
Correction Factor (260 nm)	0.05
Correction Factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	200000¹
Excitation (nm)	587
Emission (nm)	603
Quantum yield	0.53¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501