iFluor® 488 goat anti-rabbit IgG (H+L) Cross Adsorbed

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 488 goat anti-rabbit IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~491 nm and ~514 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 488 goat anti-rabbit IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 488 goat anti-mouse IgG (H+L)	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 goat anti-mouse IgG (H+L) Cross Adsorbed	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 350 goat anti-rabbit IgG (H+L)	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 405 goat anti-rabbit IgG (H+L)	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 514 goat anti-rabbit IgG (H+L)	511	527	75000¹	0.83¹	0.265	0.116
iFluor® 532 goat anti-rabbit IgG (H+L)	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 555 goat anti-rabbit IgG (H+L)	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 594 goat anti-rabbit IgG (H+L)	587	603	200000¹	0.53¹	0.05	0.04
iFluor® 633 goat anti-rabbit IgG (H+L)	640	654	250000¹	0.29¹	0.062	0.044
iFluor® 647 goat anti-rabbit IgG (H+L)	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 goat anti-rabbit IgG (H+L)	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 goat anti-rabbit IgG (H+L)	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 goat anti-rabbit IgG (H+L)	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 goat anti-rabbit IgG (H+L)	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 350 goat anti-rabbit IgG (H+L) Cross Adsorbed	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 405 goat anti-rabbit IgG (H+L) Cross Adsorbed	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 514 goat anti-rabbit IgG (H+L) Cross Adsorbed	511	527	75000¹	0.83¹	0.265	0.116
iFluor® 532 goat anti-rabbit IgG (H+L) Cross Adsorbed	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 555 goat anti-rabbit IgG (H+L) Cross Adsorbed	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 594 goat anti-rabbit IgG (H+L) Cross Adsorbed	587	603	200000¹	0.53¹	0.05	0.04
iFluor® 633 goat anti-rabbit IgG (H+L) Cross Adsorbed	640	654	250000¹	0.29¹	0.062	0.044
iFluor® 647 goat anti-rabbit IgG (H+L) Cross Adsorbed	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 goat anti-rabbit IgG (H+L) Cross Adsorbed	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 goat anti-rabbit IgG (H+L) Cross Adsorbed	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 goat anti-rabbit IgG (H+L) Cross Adsorbed	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 goat anti-rabbit IgG (H+L) Cross Adsorbed	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 546 goat anti-rabbit IgG (H+L)	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 546 goat anti-rabbit IgG (H+L) Cross Adsorbed	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 568 goat anti-rabbit IgG (H+L)	568	587	100000¹	0.57¹	0.34	0.15
iFluor® 568 goat anti-rabbit IgG (H+L) Cross Adsorbed	568	587	100000¹	0.57¹	0.34	0.15
iFluor® 800 goat anti-rabbit IgG (H+L)	801	820	250000¹	0.11¹	0.03	0.08
iFluor® 800 goat anti-rabbit IgG (H+L) Cross Adsorbed	801	820	250000¹	0.11¹	0.03	0.08
iFluor® 810 goat anti-rabbit IgG (H+L)	811	822	250000¹	0.05¹	0.09	0.15
iFluor® 810 goat anti-rabbit IgG (H+L) Cross Adsorbed	811	822	250000¹	0.05¹	0.09	0.15
iFluor® 820 goat anti-rabbit IgG (H+L)	822	850	250000¹		0.11	0.16
iFluor® 820 goat anti-rabbit IgG (H+L) Cross Adsorbed	822	850	250000¹		0.11	0.16
iFluor® 840 goat anti-rabbit IgG (H+L)	836	879	200000¹	-	0.2	0.09
iFluor® 840 goat anti-rabbit IgG (H+L) Cross Adsorbed	836	879	200000¹	-	0.2	0.09
iFluor® 860 goat anti-rabbit IgG (H+L)	853	878	250000¹		0.1	0.14
iFluor® 860 goat anti-rabbit IgG (H+L) Cross Adsorbed	853	878	250000¹		0.1	0.14
Show More (31)

Citations

View all 24 citations: Citation Explorer

Macrophage P2Y12 regulates iron transport and its inhibition protects against atherosclerosis
Authors: Hu, Yang-Xi and You, Hong-Min and Bai, Mei-Rong and Yue, Wen-Heng and Li, Fang-Fang and Hu, Bo-Wen and Chen, Ya-Sha and Shen, Xiang-Yu and Wu, Yue and Wang, Jia-Mei and others,
Journal: Journal of Advanced Research (2024)

Maresin1 Inhibits Ferroptosis via the Nrf2/SLC7A11/GPX4 Pathway to Protect Against Sepsis-Induced Acute Liver Injury
Authors: Guo, Yongjing and Chen, Huimin and Sun, Jian and Zhang, Jingxiao and Yin, Yongjie
Journal: Journal of Inflammation Research (2024): 11041--11053

The Effect of Caffeine Exposure on Sleep Patterns in Zebrafish Larvae and Its Underlying Mechanism
Authors: Wei, Yuanzheng and Miao, Zongyu and Ye, Huixin and Wu, Meihui and Wei, Xinru and Zhang, Yu and Cai, Lei
Journal: Clocks \& Sleep (2024): 749--763

NPR1 promotes cisplatin resistance by inhibiting PARL-mediated mitophagy-dependent ferroptosis in gastric cancer
Authors: Wu, Chengwei and Wang, Song and Huang, Tao and Xi, Xinran and Xu, Lishuai and Wang, Jiawei and Hou, Yinfen and Xia, Yabin and Xu, Li and Wang, Luman and others,
Journal: Cell Biology and Toxicology (2024): 93

Aspirin alleviates fibronectin-induced preeclampsia phenotypes in a mouse model and reverses fibronectin-mediated trophoblast invasiveness under hypoxia by regulating ciliogenesis and Akt and MAPK signaling
Authors: Tsai, Pei-Yin and Lee, Chih-I and Tam, Hoi-Lam and Su, Mei-Tsz
Journal: Biochemical Pharmacology (2024): 116423

References

View all 191 references: Citation Explorer

Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers
Authors: Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.
Journal: Oncotarget (2011): 728

Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding
Authors: Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.
Journal: Med Clin (Barc) (2011): 668

Falsely elevated tacrolimus levels caused by immunoassay interference secondary to beta-galactosidase antibodies in an infected liver transplant recipient
Authors: Knorr JP, Grewal KS, Balasubramanian M, Young N, Zaki R, Khanmoradi K, Araya V, Ortiz J.
Journal: Pharmacotherapy (2010): 954

Prevalence of anti-Epstein-Barr virus antibodies in children and adolescents with secondary immunodeficiency
Authors: Buckova A., undefined
Journal: Epidemiol Mikrobiol Imunol (2010): 133

Analysis of the effectiveness of reused primary and secondary antibodies in Western blotting analysis
Authors: Boonrod K, Roth B, Leong Ngar S, Krczal G.
Journal: Anal Biochem (2010): 124

Page updated on December 17, 2024

Ordering information

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Unit size	200 ug 1 mg
Catalog Number	1667816828
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Physical properties

Molecular weight	~150000
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501