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AAT Bioquest

Anti-Rabbit Secondary Antibodies

Anti-rabbit secondary antibodies are affinity-purified polyclonal antibodies specific for rabbit immunoglobulins (e.g., class, subclasses, or fragments). They are commonly used in the detection of a specific target to which a rabbit primary antibody is first bound. This indirect detection scheme provides its user with a significant increase in sensitivity due to the signal amplification of multiple secondary antibodies binding to a single primary antibody. Secondary antibodies conjugated to fluorescent dyes also provide greater flexibility in multilabeling experiments. The large number of available fluorescent dyes makes it easy to design multicolor labeling panels with minimal spillover.

AAT Bioquest anti-rabbit secondary antibodies are produced in goats and are specific for the isotype immunoglobulin G (IgG), heavy and light chains. They have been validated for use in various types of immunoassays, including ELISA, immunohistochemistry (IHC), immunoprecipitation (IP), Western blot (WB), immunoelectrophoresis (IE), time-resolved FRET, fluorescence microscopy, and flow cytometry.

 

 

iFluor® Goat Anti-Rabbit Secondaries


Tubulin Stain
HeLa cells were labeled using rabbit anti-tubulin antibody followed by detection with iFluor® 594 goat anti-rabbit IgG (H+L). Nuclei were costained with DAPI and F-actin with Phalloidin-iFluor® 488 conjugate.
Goat anti-rabbit secondary antibodies conjugated to iFluor® dyes offer exceptional brightness and photostability, outperforming other spectrally similar secondaries, such as Alexa Fluor®-, FITC-, and Cyanine-labeled secondaries. Cross-adsorbed secondary antibodies are also available for improved specificity, greater sensitivity, and lower background in multicolor labeling experiments or in IHC experiments on samples with abundant amounts of endogenous IgG. Cross-adsorption of secondary antibodies via affinity purification eliminates cross-reactivity, improves antibody specificity, and minimizes non-specific background.

Available Goat Anti-Rabbit Antibodies


 

Table 1. Anti-rabbit secondary antibodies.

Product
Host
Target Species
Conjugate
Ex (nm)
Em (nm)
Spectrum
Application
Size
Cat No.
Biotinylated goat anti-rabbit IgG (H+L)GoatRabbitBiotinWB, IHC/ICC/IF, ELISA, Flow1 mg16794
Goat anti-rabbit IgG (H+L) AgaroseGoatRabbitAgaroseIP, AP10 mg55015
HRP-labeled goat anti-rabbit IgG (H+L)GoatRabbitHRPWB, IHC/ICC/IF, ELISA, IE1 mg16793
MegaWox™ PolyHRP-goat anti-rabbit IgGGoatRabbitpolyHRPWB, IHC/ICC/IF, ELISA, IE1 mg11037
iFluor® 350 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 350345450IHC/ICC/IF, Flow200 µg16600
iFluor® 350 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 350345450IHC/ICC/IF, Flow1 mg16795
iFluor® 350 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 350345450IHC/ICC/IF, Flow200 µg16670
iFluor® 350 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 350345450IHC/ICC/IF, Flow1 mg16825
iFluor® 405 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 405403427IHC/ICC/IF, Flow200 µg16604
iFluor® 405 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 405403427IHC/ICC/IF, Flow1 mg16796
iFluor® 405 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 405403427IHC/ICC/IF, Flow200 µg16674
iFluor® 405 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 405403427IHC/ICC/IF, Flow1 mg16826
iFluor® 488 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 488491516IHC/ICC/IF, Flow200 µg16608
iFluor® 488 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 488491516IHC/ICC/IF, Flow1 mg16800
iFluor® 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 488491516IHC/ICC/IF, Flow200 µg16678
iFluor® 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 488491516IHC/ICC/IF, Flow1 mg16828
iFluor® 514 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 514528555IHC/ICC/IF, Flow200 µg16612
iFluor® 514 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 514528555IHC/ICC/IF, Flow1 mg16801
iFluor® 514 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 514528555IHC/ICC/IF, Flow200 µg16682
iFluor® 514 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 514528555IHC/ICC/IF, Flow1 mg16829
iFluor® 532 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 532537560ICC/IF, Flow200 µg16616
iFluor® 532 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 532537560ICC/IF, Flow1 mg16802
iFluor® 532 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 532537560ICC/IF, Flow200 µg16686
iFluor® 532 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 532537560ICC/IF, Flow1 mg16830
iFluor® 546 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 546541557IHC/ICC/IF, Flow200 µg16618
iFluor® 546 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 546541557IHC/ICC/IF, Flow200 µg16688
iFluor® 555 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 555557570IHC/ICC/IF, Flow200 µg16620
iFluor® 555 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 555557570IHC/ICC/IF, Flow1 mg16803
iFluor® 555 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 555557570IHC/ICC/IF, Flow200 µg16690
iFluor® 555 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 555557570IHC/ICC/IF, Flow1 mg16831
iFluor® 568 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 568568587IHC/ICC/IF, Flow200 µg16622
iFluor® 568 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 568568587IHC/ICC/IF, Flow1 mg16804
iFluor® 568 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 568568587IHC/ICC/IF, Flow200 µg16692
iFluor® 568 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 568568587IHC/ICC/IF, Flow1 mg16832
iFluor® 594 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 594588604IHC/ICC/IF, Flow200 µg16628
iFluor® 594 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 594588604IHC/ICC/IF, Flow1 mg16806
iFluor® 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 594588604IHC/ICC/IF, Flow200 µg16698
iFluor® 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 594588604IHC/ICC/IF, Flow1 mg16833
iFluor® 633 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 633640654ICC/IF, Flow200 µg16638
iFluor® 633 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 633640654ICC/IF, Flow1 mg16808
iFluor® 633 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 633640654ICC/IF, Flow200 µg16704
iFluor® 633 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 633640654ICC/IF, Flow1 mg16835
iFluor® 647 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 647656670ICC/IF, Flow200 µg16642
iFluor® 647 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 647656670ICC/IF, Flow1 mg16809
iFluor® 647 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 647656670ICC/IF, Flow200 µg16710
iFluor® 647 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 647656670ICC/IF, Flow1 mg16837
iFluor® 680 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 680684701ICC/IF, Flow200 µg16646
iFluor® 680 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 680684701ICC/IF, Flow1 mg16810
iFluor® 680 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 680684701ICC/IF, Flow200 µg16712
iFluor® 680 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 680684701ICC/IF, Flow1 mg16838
iFluor® 700 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 700690713ICC/IF, Flow200 µg16652
iFluor® 700 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 700690713ICC/IF, Flow1 mg16811
iFluor® 700 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 700690713ICC/IF, Flow200 µg16714
iFluor® 700 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 700690713ICC/IF, Flow1 mg16839
iFluor® 750 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 750757779ICC/IF, Flow200 µg16660
iFluor® 750 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 750757779ICC/IF, Flow1 mg16813
iFluor® 750 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 750757779ICC/IF, Flow200 µg16720
iFluor® 750 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 750757779ICC/IF, Flow1 mg16842
iFluor® 790 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 790787812WB, ICC/IF, Flow200 µg16661
iFluor® 790 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 790787812WB, ICC/IF, Flow1 mg16815
iFluor® 790 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 790787812WB, ICC/IF, Flow200 µg16721
iFluor® 790 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 790787812WB, ICC/IF, Flow1 mg16843
iFluor® 800 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 800801820WB, ICC/IF, Flow200 µg48050
iFluor® 800 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 800801820WB, ICC/IF, Flow1 mg48051
iFluor® 800 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 800801820WB, ICC/IF, Flow200 µg48052
iFluor® 800 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 800801820WB, ICC/IF, Flow1 mg48053
iFluor® 810 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 810811822WB, ICC/IF, Flow200 µg48054
iFluor® 810 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 810811822WB, ICC/IF, Flow1 mg48055
iFluor® 810 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 810811822WB, ICC/IF, Flow200 µg48056
iFluor® 810 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 810811822WB, ICC/IF, Flow1 mg48057
iFluor® 820 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 820822850WB, ICC/IF, Flow200 µg48058
iFluor® 820 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 820822850WB, ICC/IF, Flow1 mg48059
iFluor® 820 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 820822850WB, ICC/IF, Flow200 µg48060
iFluor® 820 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 820822850WB, ICC/IF, Flow1 mg48061
iFluor® 840 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 840836879WB, ICC/IF, Flow200 µg48062
iFluor® 840 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 840836879WB, ICC/IF, Flow1 mg48063
iFluor® 840 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 840836879WB, ICC/IF, Flow200 µg48064
iFluor® 840 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 840836879WB, ICC/IF, Flow1 mg48065
iFluor® 860 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 860853878WB, ICC/IF, Flow200 µg48066
iFluor® 860 goat anti-rabbit IgG (H+L)GoatRabbitiFluor® 860853878WB, ICC/IF, Flow1 mg48067
iFluor® 860 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 860853878WB, ICC/IF, Flow200 µg48068
iFluor® 860 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitiFluor® 860853878WB, ICC/IF, Flow1 mg48069
trFluor™ EU goat anti-rabbit IgG (H+L)GoatRabbittrFluor™ Eu346617WB, ELISA, TR-FRET100 µ16668
trFluor™ EU goat anti-rabbit IgG (H+L)GoatRabbittrFluor™ Eu346617WB, ELISA, TR-FRET1 mg16820
trFluor™ EU goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbittrFluor™ Eu346617WB, ELISA, TR-FRET100 µ16725
trFluor™ EU goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbittrFluor™ Eu346617WB, ELISA, TR-FRET1 mg16847
trFluor™ Tb goat anti-rabbit IgG (H+L)GoatRabbittrFluor™ Tb333544WB, ELISA, TR-FRET100 µg16669
trFluor™ Tb goat anti-rabbit IgG (H+L)GoatRabbittrFluor™ Tb333544WB, ELISA, TR-FRET1 mg16821
trFluor™ Tb goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbittrFluor™ Tb333544WB, ELISA, TR-FRET100 µg16726
trFluor™ Tb goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbittrFluor™ Tb333544WB, ELISA, TR-FRET1 mg16848
FITC goat anti-rabbit IgG (H+L)GoatRabbitFITC491516ICC/IF, Flow1 mg16868
FITC goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitFITC491516ICC/IF, Flow1 mg16876
Texas Red® goat anti-rabbit IgG (H+L)GoatRabbitTexas Red®586603IHC/ICC/IF, Flow1 mg16869
Texas Red® goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitTexas Red®586603IHC/ICC/IF, Flow1 mg16877
Cy3® goat anti-rabbit IgG (H+L)GoatRabbitCy3®555569ICC/IF, Flow1 mg16870
Cy3® goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitCy3®555569ICC/IF, Flow1 mg16878
Cy5® goat anti-rabbit IgG (H+L)GoatRabbitCy5®651670ICC/IF, Flow1 mg16871
Cy5® goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitCy5®651670ICC/IF, Flow1 mg16879
Cy7® goat anti-rabbit IgG (H+L)GoatRabbitCy7®756779ICC/IF, Flow1 mg16872
Cy7® goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitCy7®756779ICC/IF, Flow1 mg16880
APC-Cy7® tandem-labeled goat anti-rabbit IgG (H+L)GoatRabbitAPC-Cy7® Tandem651779ICC/IF, Flow1 mg16873
XFD350 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitXFD350 (same as Alexa Fluor 350)343441IHC/ICC/IF, Flow1 mg16395
XFD488 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitXFD488 (same as Alexa Fluor 488)499520IHC/ICC/IF, Flow1 mg16398
XFD594 goat anti-rabbit IgG (H+L) *Cross Adsorbed*GoatRabbitXFD594 (same as Alexa Fluor 594)590618IHC/ICC/IF, Flow1 mg16404