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AAT Bioquest

iFluor® 750 hydrazide

AAT Bioquest's iFluor® dyes are developed for labeling proteins, particularly antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. Our iFluor® 750 dyes have fluorescence excitation and emission maxima of ~757 nm and ~779 nm, respectively, with good photostability. Our in-house comparable studies indicated that our iFluor® 750 dyes are significantly brighter than the corresponding Cy7® and Alexa Fluor® 750. These spectral characteristics make them a superior alternative to Cy7® and Alexa Fluor® 750 (Cy7® and Alexa Fluor® are the trademarks of GE Healthcare and Invitrogen). iFluor® 780 conjugates have been widely used in fluorescence animal imaging applications. The iFluor® 750 hydrazide is reasonably stable and shows good reactivity and selectivity with the carbonyl group (such as aldehyde).

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of iFluor® 750 hydrazide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM87.176 µL435.878 µL871.756 µL4.359 mL8.718 mL
5 mM17.435 µL87.176 µL174.351 µL871.756 µL1.744 mL
10 mM8.718 µL43.588 µL87.176 µL435.878 µL871.756 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350 hydrazide3454502000010.9510.830.23
iFluor® 488 hydrazide4915167500010.910.210.11
iFluor® 555 hydrazide55757010000010.6410.230.14
iFluor® 647 hydrazide65667025000010.2510.030.03
iFluor® 680 hydrazide68470122000010.2310.0970.094
iFluor® 700 hydrazide69071322000010.2310.090.04
iFluor® 790 hydrazide78781225000010.1310.10.09
iFluor® 405 hydrazide4034273700010.9110.480.77
iFluor® 750 Styramide *Superior Replacement for Alexa Fluor 750 tyramide*75777927500010.1210.0440.039

Citations

View all 4 citations: Citation Explorer
Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153--0769
Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: Proceedings of the National Academy of Sciences (2016): E2011--E2018
Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer
Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766
Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211
Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262
Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119
Page updated on October 28, 2024

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Catalog Number1088
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Physical properties

Molecular weight

1147.11

Solvent

Water

Spectral properties

Correction Factor (260 nm)

0.044

Correction Factor (280 nm)

0.039

Correction Factor (565 nm)

0.0250

Correction Factor (650 nm)

0.1413

Extinction coefficient (cm -1 M -1)

2750001

Excitation (nm)

757

Emission (nm)

779

Quantum yield

0.121

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Fluorescent dye hydrazine derivatives are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester). Fluorescent dye hydrazine derivatives are also useful for tracing neurons.
Fluorescent dye hydrazine derivatives are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester). Fluorescent dye hydrazine derivatives are also useful for tracing neurons.
Fluorescent dye hydrazine derivatives are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester). Fluorescent dye hydrazine derivatives are also useful for tracing neurons.