iFluor® 350 hydrazide

Name: iFluor® 350 hydrazide
Brand: AAT Bioquest
SKU: 1080
Price: 227 USD
Availability: InStock

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 350 dyes have fluorescence excitation and emission maxima of ~345 nm and ~450 nm respectively. These spectral characteristics make them an excellent alternative to AMCA and Alexa Fluor® 350 labeling dye (Alexa Fluor® is the trademark of Invitrogen). iFluor® 350 hydrazide is reasonably stable and shows good reactivity and selectivity with a carbonyl group (such as aldehyde).

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of iFluor® 350 hydrazide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	125.999 µL	629.993 µL	1.26 mL	6.3 mL	12.6 mL
5 mM	25.2 µL	125.999 µL	251.997 µL	1.26 mL	2.52 mL
10 mM	12.6 µL	62.999 µL	125.999 µL	629.993 µL	1.26 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 488 hydrazide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 hydrazide	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 647 hydrazide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 hydrazide	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 hydrazide	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 hydrazide	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 hydrazide	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 405 hydrazide	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 350 Styramide Superior Replacement for Alexa Fluor 350 tyramide	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 350 Tyramide	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 350-dUTP 1 mM in TE Buffer (pH 7.5)	345	450	20000¹	0.95¹	0.83	0.23
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Citations

View all 3 citations: Citation Explorer

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119

Page updated on October 28, 2024

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Catalog Number	1080
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Telephone	1-800-990-8053
Fax	1-800-609-2943
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Physical properties

Molecular weight	793.66
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.83
Correction Factor (280 nm)	0.23
Extinction coefficient (cm ^-1 M ^-1)	20000¹
Excitation (nm)	345
Emission (nm)	450
Quantum yield	0.95¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501