iFluor® 488 hydrazide

Name: iFluor® 488 hydrazide
Brand: AAT Bioquest
SKU: 1082
Price: 227 USD
Availability: InStock

Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor® 488 dyes are far superior to conjugates of fluorescein derivatives such as FITC. iFluor® 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor® 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor® 488 hydrazide is reasonably stable and shows good reactivity and selectivity with a carbonyl group (such as aldehyde). This iFluor® 488 has spectral properties and reactivity similar to Alexa Fluor® 488 hydrazide ( Alexa Fluor® is the trademark of Invitrogen).

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of iFluor® 488 hydrazide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	112.288 µL	561.438 µL	1.123 mL	5.614 mL	11.229 mL
5 mM	22.458 µL	112.288 µL	224.575 µL	1.123 mL	2.246 mL
10 mM	11.229 µL	56.144 µL	112.288 µL	561.438 µL	1.123 mL

Molarity calculator

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Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 350 hydrazide	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 555 hydrazide	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 647 hydrazide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 hydrazide	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 hydrazide	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 hydrazide	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 hydrazide	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 488 tyramide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 405 hydrazide	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 488 Styramide Superior Replacement for Alexa Fluor 488 tyramide and Opal 520	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 TCO	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 Tetrazine	491	516	75000¹	0.9¹	0.21	0.11
iFluor®488-dUTP 1 mM in TE Buffer (pH 7.5)	491	516	75000¹	0.9¹	0.21	0.11
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Citations

View all 23 citations: Citation Explorer

Homoscleromorpha): A New Convenient Model for Sponge Cell and Evolutionary Developmental Biology
Authors: Rocher, Caroline and Vernale, Am{\'e}lie and Fierro-Consta{\'\i}n, Laura and S{\'e}journ{\'e}, Nina and Chenesseau, Sandrine and Marschal, Christian and Issartel, Julien and Le Goff, Emilie and Stroebel, David and Jouvion, Julie and others,
Journal: (2024)

Vibrational Markers of a Model Circulating Metastatic Cells LLC-R9
Authors: Gnatyuk, Olena and Kolesnyk, Denys and Voitsitskyi, Taras and Karakhim, Sergiy and Nikolenko, Andriy and Dementjev, Andrej and Solyanik, Galina and Dovbeshko, Galyna
Journal: Spectroscopy Journal (2024): 306--321

IKIP downregulates THBS1/FAK signaling to suppress migration and invasion by glioblastoma cells
Authors: Zhu, Zhaoying and Hu, Yanjia and Ye, Feng and Teng, Haibo and You, Guoliang and Zeng, Yunhui and Tian, Meng and Xu, Jianguo and Li, Jin and Liu, Zhiyong and others,
Journal: Oncology Research (2024): 1173

Pharmacological inhibition of RAS overcomes FLT3 inhibitor resistance in FLT3-ITD+ AML through AP-1 and RUNX1
Authors: Coleman, Daniel JL and Keane, Peter and Chin, Paulynn S and Ames, Luke and Kellaway, Sophie and Blair, Helen and Khan, Naeem and Griffin, James and Holmes, Elizabeth and Maytum, Alexander and others,
Journal: iScience (2024)

A high-throughput DNA analysis method based on isothermal amplification on a suspension microarray for detecting mpox virus and viruses with comparable symptoms
Authors: Zhang, Liming and Liu, Jieyu and Huang, Shisi and Zeng, Wentao and Li, Li and Fan, Xihao and Lu, Zhuoxuan
Journal: Analytica Chimica Acta (2024): 342416

References

View all 49 references: Citation Explorer

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211

Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766

Page updated on December 17, 2024

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Catalog Number	1082
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Physical properties

Molecular weight	890.57
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501