iFluor®488-dUTP 1 mM in TE Buffer (pH 7.5)

The dye-modified deoxyuridine 5'-triphosphates are one of the most common methods to produce dye-labelled DNA via the conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This iFluor 488-dUTP conjugate is used as a green fluorescence color with SpectrumGreen™ filter set (SpectrumGreen™ is the trademark of Vysis). Compared to the commonly used green color probes (e.g., Fluorescein-12-dUTP), iFluor 488 provides much brighter signal that has much photostability and is not affected by pH.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 488 maleimide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 amine	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 hydrazide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 tyramide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 azide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 alkyne	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 Styramide Superior Replacement for Alexa Fluor 488 tyramide and Opal 520	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 TCO	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 Tetrazine	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 440-dUTP 1 mM in TE Buffer (pH 7.5)	434	480	40000¹	0.67¹	0.352	0.229
iFluor® 560-dUTP 1 mM in TE Buffer (pH 7.5)	560	571	120000¹	0.57¹	0.0482	0.069
iFluor® 350-dUTP 1 mM in TE Buffer (pH 7.5)	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 532-dUTP 1 mM in TE Buffer (pH 7.5)	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 570-dUTP 1 mM in TE Buffer (pH 7.5)	557	570	120000¹	0.58¹	-	-
XFD™488-dUTP 1 mM in TE Buffer (pH 7.5)	499	520	71000	0.92¹	0.30	0.11
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References

View all 23 references: Citation Explorer

Anti-proliferative effect of Fe(III) complexed with 1-(2-hydroxy-3-methoxybenzaldehyde)-4-aminosalicylhydrazone in HepG2 cells.
Authors: Fukushima, Takeshi and Taniguchi, Erina and Yamada, Hiroshi and Kato, Kiyomasa and Shimizu, Ayako and Nishiguchi, Yoshikazu and Onozato, Mayu and Ichiba, Hideaki and Azuma, Yutaro
Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2015): 669-77

Localisation of abundant and organ-specific genes expressed in Rosa hybrida leaves and flower buds by direct in situ RT-PCR.
Authors: Jedrzejuk, Agata and Mibus, Heiko and Serek, Margrethe
Journal: TheScientificWorldJournal (2012): 609597

SNP genotyping using microsphere-linked PNA and flow cytometric detection.
Authors: Rockenbauer, Eszter and Petersen, Kenneth and Vogel, Ulla and Bolund, Lars and Kølvraa, Steen and Nielsen, Kirsten Vang and Nexø, Bjørn A
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 80-6

[Comparative genomic hybridization analysis of nasopharyngeal carcinoma drug-resistant cell CNE2/DDP and its parent cell CNE2].
Authors: Jiang, Run-De and Hu, Liang and Guan, Xin-Yuan and Zhang, Li-Xin and Yue, Wen and Cen, Xin-Tang and Li, Chun-Hai
Journal: Ai zheng = Aizheng = Chinese journal of cancer (2004): 386-90

Flow cytometric detection of beta-D-glucuronidase gene in wild-type bacterial cells using in-situ PCR.
Authors: Sachidanandham, Ramaiah and Gin, Karina Yew-Hoong
Journal: Biotechnology and bioengineering (2003): 127-33

Page updated on November 20, 2024

Ordering information

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Unit size	25 nmoles 100 nmoles
Catalog Number	1703917044
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Physical properties

Molecular weight	1152.83
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501