iFluor® 488 TCO
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 945.16 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: iFluor® Dyes and Kits
Molecular weight 945.16 | Correction Factor (260 nm) 0.21 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 491 | Emission (nm) 516 | Quantum yield 0.91 |
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 488-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. The conjugates prepared with iFluor® 488 dyes are far superior to those of fluorescein derivatives such as FITC. iFluor® 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor® 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor® 488 has spectral properties similar to Alexa Fluor® 488. In some cases, it demonstrates brighter signals (Alexa Fluor® is the trademark of Invitrogen).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 488 TCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 105.802 µL | 529.011 µL | 1.058 mL | 5.29 mL | 10.58 mL |
5 mM | 21.16 µL | 105.802 µL | 211.604 µL | 1.058 mL | 2.116 mL |
10 mM | 10.58 µL | 52.901 µL | 105.802 µL | 529.011 µL | 1.058 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fifluor_488.png&w=2048&q=50)
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fifluor_488.png&w=2048&q=50)
Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 488 maleimide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 488 amine | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 488 hydrazide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 488 tyramide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 488 azide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 488 alkyne | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 488 Styramide *Superior Replacement for Alexa Fluor 488 tyramide and Opal 520* | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555 TCO | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 594 TCO | 587 | 603 | 2000001 | 0.531 | 0.05 | 0.04 |
Show More (3) |
Images
![The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 488-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fifluor-488-tco%2Ffigure-for-ifluor-488-tco_FKI7L.jpg&w=3840&q=75)
Figure 1. The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 488-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.
Citations
View all 1 citations: Citation Explorer
CD95/Fas protects triple negative breast cancer from anti-tumor activity of NK cells
Authors: Qadir, Abdul S and Gu{\'e}gan, Jean Philippe and Ginestier, Christophe and Chaibi, Assia and Bessede, Alban and Charafe-Jauffret, Emmanuelle and Macario, Manon and Lavou{\'e}, Vincent and de la Motte Rouge, Thibault and Law, Calvin and others,
Journal: Iscience (2021): 103348
Authors: Qadir, Abdul S and Gu{\'e}gan, Jean Philippe and Ginestier, Christophe and Chaibi, Assia and Bessede, Alban and Charafe-Jauffret, Emmanuelle and Macario, Manon and Lavou{\'e}, Vincent and de la Motte Rouge, Thibault and Law, Calvin and others,
Journal: Iscience (2021): 103348
References
View all 50 references: Citation Explorer
A Systematic Study of Coumarin-Tetrazine Light-Up Probes for Bioorthogonal Fluorescence Imaging.
Authors: Galeta, Juraj and Dzijak, Rastislav and Obořil, Jan and Dračínský, Martin and Vrabel, Milan
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)
Authors: Galeta, Juraj and Dzijak, Rastislav and Obořil, Jan and Dračínský, Martin and Vrabel, Milan
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)
Bioorthogonal Tetrazine Carbamate Cleavage by Highly Reactive trans-Cyclooctene.
Authors: van Onzen, Arthur H A M and Versteegen, Ron M and Hoeben, Freek J M and Filot, Ivo A W and Rossin, Raffaella and Zhu, Tong and Wu, Jeremy and Hudson, Peter J and Janssen, Henk M and Ten Hoeve, Wolter and Robillard, Marc S
Journal: Journal of the American Chemical Society (2020): 10955-10963
Authors: van Onzen, Arthur H A M and Versteegen, Ron M and Hoeben, Freek J M and Filot, Ivo A W and Rossin, Raffaella and Zhu, Tong and Wu, Jeremy and Hudson, Peter J and Janssen, Henk M and Ten Hoeve, Wolter and Robillard, Marc S
Journal: Journal of the American Chemical Society (2020): 10955-10963
Clickable modular polysaccharide nanoparticles for selective cell-targeting.
Authors: Peuler, Kevin and Dimmitt, Nathan and Lin, Chien-Chi
Journal: Carbohydrate polymers (2020): 115901
Authors: Peuler, Kevin and Dimmitt, Nathan and Lin, Chien-Chi
Journal: Carbohydrate polymers (2020): 115901
Facile Discovery and Quantification of Isonitrile Natural Products via Tetrazine-Based Click Reactions.
Authors: Huang, Yao-Bing and Cai, Wenlong and Del Rio Flores, Antonio and Twigg, Frederick F and Zhang, Wenjun
Journal: Analytical chemistry (2020): 599-602
Authors: Huang, Yao-Bing and Cai, Wenlong and Del Rio Flores, Antonio and Twigg, Frederick F and Zhang, Wenjun
Journal: Analytical chemistry (2020): 599-602
In Vivo Imaging of Click-Crosslinked Hydrogel Depots Following Intratympanic Injection.
Authors: Ju, Hyeon Jin and Park, Mina and Park, Ji Hoon and Shin, Gi Ru and Choi, Hak Soo and Suh, Myung-Whan and Kim, Moon Suk
Journal: Materials (Basel, Switzerland) (2020)
Authors: Ju, Hyeon Jin and Park, Mina and Park, Ji Hoon and Shin, Gi Ru and Choi, Hak Soo and Suh, Myung-Whan and Kim, Moon Suk
Journal: Materials (Basel, Switzerland) (2020)
Design, Synthesis, Conjugation, and Reactivity of Novel trans,trans-1,5-Cyclooctadiene-Derived Bioorthogonal Linkers.
Authors: Longo, Beatrice and Zanato, Chiara and Piras, Monica and Dall'Angelo, Sergio and Windhorst, Albert D and Vugts, Danielle J and Baldassarre, Massimiliano and Zanda, Matteo
Journal: Bioconjugate chemistry (2020)
Authors: Longo, Beatrice and Zanato, Chiara and Piras, Monica and Dall'Angelo, Sergio and Windhorst, Albert D and Vugts, Danielle J and Baldassarre, Massimiliano and Zanda, Matteo
Journal: Bioconjugate chemistry (2020)
Injectable Click Chemistry-based Bioadhesives for Accelerated Wound Closure.
Authors: Li, Shuxin and Zhou, Jun and Huang, YiHui and Roy, Joyita and Zhou, Ning and Yum, Kyungsuk and Sun, Xiankai and Tang, Liping
Journal: Acta biomaterialia (2020): 95-104
Authors: Li, Shuxin and Zhou, Jun and Huang, YiHui and Roy, Joyita and Zhou, Ning and Yum, Kyungsuk and Sun, Xiankai and Tang, Liping
Journal: Acta biomaterialia (2020): 95-104
Conditionally Activatable Visible-Light Photocages.
Authors: Bojtár, Márton and Németh, Krisztina and Domahidy, Farkas and Knorr, Gergely and Verkman, András and Kállay, Mihály and Kele, Péter
Journal: Journal of the American Chemical Society (2020)
Authors: Bojtár, Márton and Németh, Krisztina and Domahidy, Farkas and Knorr, Gergely and Verkman, András and Kállay, Mihály and Kele, Péter
Journal: Journal of the American Chemical Society (2020)
Systematic Screening of Different Polyglycerin-Based Dienophile Macromonomers for Efficient Nanogel Formation through IEDDA Inverse Nanoprecipitation.
Authors: Oehrl, Alexander and Schötz, Sebastian and Haag, Rainer
Journal: Macromolecular rapid communications (2020): e1900510
Authors: Oehrl, Alexander and Schötz, Sebastian and Haag, Rainer
Journal: Macromolecular rapid communications (2020): e1900510
Hybrid Imaging Agents for Pretargeting Applications Based on Fusarinine C-Proof of Concept.
Authors: Summer, Dominik and Petrik, Milos and Mayr, Sonja and Hermann, Martin and Kaeopookum, Piriya and Pfister, Joachim and Klingler, Maximilian and Rangger, Christine and Haas, Hubertus and Decristoforo, Clemens
Journal: Molecules (Basel, Switzerland) (2020)
Authors: Summer, Dominik and Petrik, Milos and Mayr, Sonja and Hermann, Martin and Kaeopookum, Piriya and Pfister, Joachim and Klingler, Maximilian and Rangger, Christine and Haas, Hubertus and Decristoforo, Clemens
Journal: Molecules (Basel, Switzerland) (2020)