iFluor® 488 alkyne

Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor® 488 dyes are far superior to conjugates of fluorescein derivatives such as FITC. iFluor® 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor® 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor® 488 alkyne dye is reasonably stable and shows good reactivity and selectivity with azide group for click chemistry applications (CuAAC). The iFluor® 488 dyes are an excellent replacement to the Alexa Fluor® dyes that have identical spectral properties (Alexa Fluor® is the trademark of Invitrogen).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 488 alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	129.212 µL	646.062 µL	1.292 mL	6.461 mL	12.921 mL
5 mM	25.842 µL	129.212 µL	258.425 µL	1.292 mL	2.584 mL
10 mM	12.921 µL	64.606 µL	129.212 µL	646.062 µL	1.292 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 647 alkyne	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 488 tyramide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 alkyne	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 488 Styramide Superior Replacement for Alexa Fluor 488 tyramide and Opal 520	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 TCO	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 Tetrazine	491	516	75000¹	0.9¹	0.21	0.11
iFluor®488-dUTP 1 mM in TE Buffer (pH 7.5)	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 790 Alkyne	787	812	250000¹	0.13¹	0.1	0.09
ATTO 488 alkyne	499	520	90000	0.80	0.22	0.09

Citations

View all 19 citations: Citation Explorer

IKIP downregulates THBS1/FAK signaling to suppress migration and invasion by glioblastoma cells
Authors: Zhu, Zhaoying and Hu, Yanjia and Ye, Feng and Teng, Haibo and You, Guoliang and Zeng, Yunhui and Tian, Meng and Xu, Jianguo and Li, Jin and Liu, Zhiyong and others,
Journal: Oncology Research (2024): 1173

Pharmacological inhibition of RAS overcomes FLT3 inhibitor resistance in FLT3-ITD+ AML through AP-1 and RUNX1
Authors: Coleman, Daniel JL and Keane, Peter and Chin, Paulynn S and Ames, Luke and Kellaway, Sophie and Blair, Helen and Khan, Naeem and Griffin, James and Holmes, Elizabeth and Maytum, Alexander and others,
Journal: iScience (2024)

A high-throughput DNA analysis method based on isothermal amplification on a suspension microarray for detecting mpox virus and viruses with comparable symptoms
Authors: Zhang, Liming and Liu, Jieyu and Huang, Shisi and Zeng, Wentao and Li, Li and Fan, Xihao and Lu, Zhuoxuan
Journal: Analytica Chimica Acta (2024): 342416

FAM81A is a postsynaptic protein that regulates the condensation of postsynaptic proteins via liquid--liquid phase separation
Authors: Kaizuka, Takeshi and Hirouchi, Taisei and Saneyoshi, Takeo and Shirafuji, Toshihiko and Collins, Mark O and Grant, Seth GN and Hayashi, Yasunori and Takumi, Toru
Journal: Plos Biology (2024): e3002006

Phosphorylation-dependent membraneless organelle fusion and fission illustrated by postsynaptic density assemblies
Authors: Wu, Haowei and Chen, Xudong and Shen, Zeyu and Li, Hao and Liang, Shiqi and Lu, Youming and Zhang, Mingjie
Journal: Molecular Cell (2023)

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119

Page updated on November 16, 2024

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Catalog Number	999
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Physical properties

Molecular weight	773.92
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501