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iFluor® 532-dUTP *1 mM in TE Buffer (pH 7.5)*
Dye-modified deoxyuridine 5'-triphosphates are widely used for the production of dye-labeled DNA via conventional enzymatic incorporation techniques, including reverse transcription, nick translation, random primed labeling, and PCR. These enzymatic fluorescence labeling methods are commonly employed in both fluorescence in situ hybridization (FISH) probes and microarray-based experiments. The iFluor® 532-dUTP conjugate, which emits yellow fluorescence, is compatible with the SpectrumGold™ filter set (SpectrumGold™ is a trademark of Vysis). Compared to Alexa Fluor® 532-labeled dNTPs, iFluor® 532 offers a significantly brighter signal, enhanced photostability, and greater resilience to pH fluctuations, maintaining stability even in higher pH conditions (e.g., pH 8-10, commonly used in DNA sequencing).
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| iFluor® 532 maleimide | 537 | 560 | 900001 | 0.681 | 0.26 | 0.16 |
| iFluor® 440-dUTP *1 mM in TE Buffer (pH 7.5)* | 434 | 480 | 400001 | 0.671 | 0.352 | 0.229 |
| iFluor®488-dUTP *1 mM in TE Buffer (pH 7.5)* | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
| iFluor® 532 Styramide | 537 | 560 | 900001 | 0.681 | 0.26 | 0.16 |
| iFluor® 560-dUTP *1 mM in TE Buffer (pH 7.5)* | 560 | 571 | 1200001 | 0.571 | 0.0482 | 0.069 |
| iFluor® 350-dUTP *1 mM in TE Buffer (pH 7.5)* | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
References
View all 50 references: Citation Explorer
Fluorescence in situ hybridization-negative intra-articular myxoid liposarcoma with complex rearrangements involving EWSR1::DDIT3 detected using nanopore sequencing.
Authors: Makise, Naohiro and Lin, Jason and Kageyama, Hajime and Takeda, Naoki and Oikawa, Mariko and Sugiyama, Takahiro and Kawana, Hidetada and Araki, Akinobu and Kinoshita, Hideyuki and Kamoda, Hiroto and Hagiwara, Yoko and Yoshida, Akihiko and Yonemoto, Tsukasa and Kawazu, Masahito and Itami, Makiko
Journal: Pathology international (2024)
Authors: Makise, Naohiro and Lin, Jason and Kageyama, Hajime and Takeda, Naoki and Oikawa, Mariko and Sugiyama, Takahiro and Kawana, Hidetada and Araki, Akinobu and Kinoshita, Hideyuki and Kamoda, Hiroto and Hagiwara, Yoko and Yoshida, Akihiko and Yonemoto, Tsukasa and Kawazu, Masahito and Itami, Makiko
Journal: Pathology international (2024)
In-situ evaluation the fluctuation of hypochlorous acid in acute liver injury mice models with a mitochondria-targeted NIR ratiometric fluorescent probe.
Authors: Liang, Yueyin and Zhang, Chunjie and Meng, Zhiyuan and Gong, Shuai and Tian, Jixiang and Li, Ruoming and Wang, Zhonglong and Wang, Shifa
Journal: Talanta (2024): 126355
Authors: Liang, Yueyin and Zhang, Chunjie and Meng, Zhiyuan and Gong, Shuai and Tian, Jixiang and Li, Ruoming and Wang, Zhonglong and Wang, Shifa
Journal: Talanta (2024): 126355
A New Peptide Nucleic Acid Fluorescence In Situ Hybridization Probe for the Specific Detection of Salmonella Species in Food Matrices.
Authors: Sousa, Mário and Rocha, Rui and Araújo, Daniela and Castro, Joana and Barbosa, Ana and Azevedo, Nuno F and Cerqueira, Laura and Almeida, Carina
Journal: Foodborne pathogens and disease (2024): 298-305
Authors: Sousa, Mário and Rocha, Rui and Araújo, Daniela and Castro, Joana and Barbosa, Ana and Azevedo, Nuno F and Cerqueira, Laura and Almeida, Carina
Journal: Foodborne pathogens and disease (2024): 298-305
Formamide denaturation of double-stranded DNA for fluorescence in situ hybridization (FISH) distorts nanoscale chromatin structure.
Authors: Shim, Anne R and Frederick, Jane and Pujadas, Emily M and Kuo, Tiffany and Ye, I Chae and Pritchard, Joshua A and Dunton, Cody L and Gonzalez, Paola Carrillo and Acosta, Nicolas and Jain, Surbhi and Anthony, Nicholas M and Almassalha, Luay M and Szleifer, Igal and Backman, Vadim
Journal: PloS one (2024): e0301000
Authors: Shim, Anne R and Frederick, Jane and Pujadas, Emily M and Kuo, Tiffany and Ye, I Chae and Pritchard, Joshua A and Dunton, Cody L and Gonzalez, Paola Carrillo and Acosta, Nicolas and Jain, Surbhi and Anthony, Nicholas M and Almassalha, Luay M and Szleifer, Igal and Backman, Vadim
Journal: PloS one (2024): e0301000
The Application of Fluorescence In Situ Hybridization in the Prescreening of Veronica Hybrids.
Authors: Park, Hye-Wan and Sevilleno, Samantha Serafin and Ha, My Khanh Tran Thi and Cabahug-Braza, Raisa Aone and Yi, Ji-Hun and Lim, Ki-Byung and Cho, Wonwoo and Hwang, Yoon-Jung
Journal: Plants (Basel, Switzerland) (2024)
Authors: Park, Hye-Wan and Sevilleno, Samantha Serafin and Ha, My Khanh Tran Thi and Cabahug-Braza, Raisa Aone and Yi, Ji-Hun and Lim, Ki-Byung and Cho, Wonwoo and Hwang, Yoon-Jung
Journal: Plants (Basel, Switzerland) (2024)
Page updated on September 24, 2024
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