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XFD™488-dUTP *1 mM in TE Buffer (pH 7.5)*
The dye-modified deoxyuridine 5'-triphosphates are one of the most common methods to produce dye-labelled DNA via the conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This XFD 488-dUTP conjugate has the same chemical structure to ChromaTide™Alexa Fluor 488-5-dUTP. It is used as a green fluorescence color with SpectrumGreen™ filter set. It provides a bright signal that has high photostability and is not affected by pH. ChromaTide™ and Alexa Fluor™ are the trademarks of ThermoFisher, and SpectrumGreen™ is the trademark of Vysis.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| iFluor®488-dUTP *1 mM in TE Buffer (pH 7.5)* | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
References
View all 6 references: Citation Explorer
Pegylated-Catalase Is Protective in Lung Ischemic Injury and Oxidative Stress.
Authors: Kim, Jung-Lye and Reader, Brenda F and Dumond, Curtis and Lee, Yonggyu and Mokadam, Nahush A and Black, Sylvester M and Whitson, Bryan A
Journal: The Annals of thoracic surgery (2021): 1019-1027
Authors: Kim, Jung-Lye and Reader, Brenda F and Dumond, Curtis and Lee, Yonggyu and Mokadam, Nahush A and Black, Sylvester M and Whitson, Bryan A
Journal: The Annals of thoracic surgery (2021): 1019-1027
Peptide amphiphile nanofiber delivery of sonic hedgehog protein to reduce smooth muscle apoptosis in the penis after cavernous nerve resection.
Authors: Bond, Christopher W and Angeloni, Nicholas L and Harrington, Daniel A and Stupp, Samuel I and McKenna, Kevin E and Podlasek, Carol A
Journal: The journal of sexual medicine (2011): 78-89
Authors: Bond, Christopher W and Angeloni, Nicholas L and Harrington, Daniel A and Stupp, Samuel I and McKenna, Kevin E and Podlasek, Carol A
Journal: The journal of sexual medicine (2011): 78-89
Visualization of heavy ion tracks by labeling 3'-OH termini of induced DNA strand breaks.
Authors: Konishi, Teruaki and Takeyasu, Akihiro and Natsume, Toshiyuki and Furusawa, Yoshiya and Hieda, Kotaro
Journal: Journal of radiation research (2011): 433-40
Authors: Konishi, Teruaki and Takeyasu, Akihiro and Natsume, Toshiyuki and Furusawa, Yoshiya and Hieda, Kotaro
Journal: Journal of radiation research (2011): 433-40
A novel screening method for competitive FRET-aptamers applied to E. coli assay development.
Authors: Bruno, John G and Carrillo, Maria P and Phillips, Taylor and Andrews, Carrie J
Journal: Journal of fluorescence (2010): 1211-23
Authors: Bruno, John G and Carrillo, Maria P and Phillips, Taylor and Andrews, Carrie J
Journal: Journal of fluorescence (2010): 1211-23
Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection.
Authors: Bruno, John G and Carrillo, Maria P and Phillips, Taylor
Journal: Journal of biomolecular techniques : JBT (2008): 109-15
Authors: Bruno, John G and Carrillo, Maria P and Phillips, Taylor
Journal: Journal of biomolecular techniques : JBT (2008): 109-15
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