XFD350 alkyne
XFD350, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor® 350 (ThermoFisher). This blue-fluorescent dye exhibits strong absorption and moderate photostability, with consistent fluorescence across a broad pH range (pH 4-11). XFD350 is effectively excited by the 350 nm line of a UV laser, and its moderate brightness and stability make it particularly advantageous for direct imaging of high-abundance targets in both microscopy and flow cytometry applications.
The alkyne derivative of XFD350 is widely used for labeling azides on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-containing molecules.
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne* | 499 | 520 | 71000 | 0.11 |
XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350* | 343 | 441 | 19000 | 0.19 |
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid* | 343 | 441 | 19000 | 0.19 |
XFD647 Alkyne | 650 | 671 | 270000 | 0.03 |
XFD350 tetrazine | 343 | 441 | 19000 | 0.19 |
XFD350 TCO | 343 | 441 | 19000 | 0.19 |
XFD405 alkyne | 401 | 421 | 35,000 | 0.70 |
XFD430 alkyne | 432 | 540 | 15,000 | 0.28 |
XFD514 alkyne | 518 | 543 | 80000 | 0.18 |
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Page updated on October 27, 2024