XFD350 alkyne

• Ex/Em: 343/441 nm
• Extinction coefficient: 19,000 cm^-1M^-1
• Reactive Group: Alkyne
• Efficient Conjugation: Click chemistry labeling of azides on peptides, antibodies, and other biomolecules
• Moderate Brightness & pH Stability: Ideal for high-abundance targets and maintains consistent fluorescence across a broad pH range (4–11)
• High Contrast: Minimizes spectral overlap with common fluorophores like fluorescein and XFD488, enhancing multiplexing and signal clarity

XFD350, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor® 350 (ThermoFisher). This blue-fluorescent dye exhibits strong absorption and moderate photostability, with consistent fluorescence across a broad pH range (pH 4-11). XFD350 is effectively excited by the 350 nm line of a UV laser, and its moderate brightness and stability make it particularly advantageous for direct imaging of high-abundance targets in both microscopy and flow cytometry applications.

The alkyne derivative of XFD350 is widely used for labeling azides on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-containing molecules.