XFD568 alkyne

• Ex/Em: 579/603 nm
• Extinction coefficient: 91,300 cm^-1M^-1
• Reactive Group: Alkyne
• Efficient Conjugation: Click chemistry labeling of azides on peptides, antibodies, and other biomolecules
• Bright & Stable: Provides high quantum yield with resilience to photobleaching and pH variations from 4 to 10
• Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies

XFD568, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 568 (ThermoFisher). This bright orange-fluorescent dye is efficiently excited by the 568 nm line of the AR-Kr mixed-gas laser and is compatible with RFP filters like Texas Red. It demonstrates excellent solubility in aqueous solutions and is pH-insensitive across a broad range (pH 4–10), ensuring reliable and stable signal generation under diverse experimental conditions. XFD568 is particularly well-suited for multicolor fluorescence microscopy, flow cytometry, and advanced imaging techniques like dSTORM. It can be conjugated to proteins at high molar ratios with minimal self-quenching, resulting in brighter conjugates. Moreover, the superior fluorescence quantum yield and photostability of XFD568 make it ideal for detecting low-abundance biological targets, enabling greater precision and sensitivity in quantitative fluorescence assays.

The alkyne derivative of XFD568 is widely used for labeling azides on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-containing molecules.