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XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne*
Product key features
- Ex/Em: 499/520 nm
- Extinction coefficient: 71,000 cm-1M-1
- Reactive Group: Alkyne
- Efficient Conjugation: Click chemistry labeling of azides on peptides, antibodies, and other biomolecules
- Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
- Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications
Product description
The fluorescent dye alkynes react with azides via a copper-catalyzed click reaction (CuAAC). This click chemistry is increasingly being used in a variety of biological applications. XFD488 alkyne has the same chemical structure to Alexa Fluor® 488 alkyne (Alexa Fluor® is the Trademark of ThermoFisher Scientific). XFD488 alkyne is reactive with terminal alkynes via a copper-catalyzed click reaction as a bio-orthogonal or biologically unique hapten for use in applications requiring signal amplification.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 129.212 µL | 646.062 µL | 1.292 mL | 6.461 mL | 12.921 mL |
| 5 mM | 25.842 µL | 129.212 µL | 258.425 µL | 1.292 mL | 2.584 mL |
| 10 mM | 12.921 µL | 64.606 µL | 129.212 µL | 646.062 µL | 1.292 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| XFD488 Hydroxylamine *Same Structure to Alexa Fluor™ 488 Hydroxylamine* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| XFD488 TCO | 499 | 520 | 73000 | 0.921 | 0.3 | 0.11 |
| XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| XFD555 alkyne | 553 | 568 | 150000 | 0.11 | 0.08 | 0.08 |
| XFD635 alkyne | 633 | 647 | 140,000 | - | - | - |
| XFD647 Alkyne | 650 | 671 | 270000 | 0.331 | - | 0.03 |
| XFD680 alkyne | 681 | 704 | 184000 | 0.361 | 0.00 | 0.05 |
| XFD700 alkyne | 696 | 719 | 192000 | 0.251 | 0.00 | 0.07 |
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References
View all 39 references: Citation Explorer
Development of new hCaM-Alexa Fluor(R) biosensors for a wide range of ligands
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep
Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)
Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601
In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12
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