Name: XFD488 TCO
Brand: AAT Bioquest
SKU: 4601
Price: 208 USD
Availability: InStock

XFD488 TCO

XFD488 TCO is a tetrazine-reactive, green fluorescent dye with an excitation/emission of 499/520 nm that is used for flow cytometry and microscopy applications.

Click Chemistry Conjugation: Provides rapid and copper-free labeling of tetrazine-modified biomolecules
Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

Schematic illustrating the strain‐promoted inverse‐electron‐demand Diels–Alder (iEDDA) reaction between a trans‐cyclooctene (TCO)–dye conjugate and a tetrazine‐modified biomolecule. The TCO–dye adduct selectively and rapidly couples with the tetrazine moiety on the biomolecule to form a stable linkage. This bioorthogonal “click chemistry” reaction is fast, selective, and well‐suited for biocompatible labeling applications.

Catalog	Size	Price	Quantity
4601	1 mg	Price

Overview

XFD488 contains the same fluorophore of Alexa Fluor® 488. It is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line. It is used for stable signal generation in imaging and flow cytometry. AF488 TCO demonstrates exceptionally fast reaction with tetrazines, the fastest kinetics ever reported for any biorthogonal reaction. It provides a convenient tool for making biological conjugates with tetrazine-modified substrates. The resulted conjugates provide the bright, green fluorescence of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher Scientific).

References

View all 50 references: Citation Explorer

Detection of Homologous Blood Transfusion in Sport Doping by Flow Cytofluorimetry: State of the Art and New Approaches to Reduce the Risk of False-Negative Results.

Authors:

Donati, Francesco and de la Torre, Xavier and Pagliarosi, Sarajane and Pirri, Daniela and Prevete, Giuliana and Botrè, Francesco

Journal:

Frontiers in sports and active living (2022): 808449

A fluorescent aptasensor for the detection of Aflatoxin B1 by graphene oxide mediated quenching and release of fluorescence.

Authors:

Setlem, Sai Keerthana and Mondal, Bhairab and Ramlal, Shylaja

Journal:

Journal of microbiological methods (2022): 106414

Introducing Cysteines into Nanobodies for Site-Specific Labeling.

Authors:

Hansen, Simon Boje and Andersen, Kasper Røjkjær

Journal:

Methods in molecular biology (Clifton, N.J.) (2022): 327-343

Dynamics of intracellular neonatal Fc receptor-ligand interactions in primary macrophages using biophysical fluorescence techniques.

Authors:

Pannek, Andreas and Houghton, Fiona J and Verhagen, Anne M and Dower, Steven K and Hinde, Elizabeth and Gleeson, Paul A

Journal:

Molecular biology of the cell (2022): ar6

Cholecystokinin-B Receptor-Targeted Nanoparticle for Imaging and Detection of Precancerous Lesions in the Pancreas.

Authors:

Smith, Jill P and Cao, Hong and Edmondson, Elijah F and Dasa, Siva Sai Krishna and Stern, Stephan T

Journal:

Biomolecules (2021)

Physical properties

Molecular weight	945.16
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.3
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92 ¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 21, 2025