XFD488 TCO

Product key features

Ex/Em: 499/520 nm
Extinction coefficient: 71,000 cm^-1M^-1
Reactive Group: TCO
Click Chemistry Conjugation: Provides rapid and copper-free labeling of tetrazine-modified biomolecules
Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

Product description

XFD488 contains the same fluorophore of Alexa Fluor® 488. It is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line. It is used for stable signal generation in imaging and flow cytometry. AF488 TCO demonstrates exceptionally fast reaction with tetrazines, the fastest kinetics ever reported for any biorthogonal reaction. It provides a convenient tool for making biological conjugates with tetrazine-modified substrates. The resulted conjugates provide the bright, green fluorescence of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher Scientific).

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD488 azide Same Structure to Alexa Fluor™ 488 azide	499	520	71000	0.92¹	0.30	0.11
XFD488 alkyne Same Structure to Alexa Fluor™ 488 alkyne	499	520	71000	0.92¹	0.30	0.11
XFD488 Hydroxylamine Same Structure to Alexa Fluor™ 488 Hydroxylamine	499	520	71000	0.92¹	0.30	0.11
XFD488 amine Same Structure to Alexa Fluor™ 488 amine	499	520	71000	0.92¹	0.30	0.11
XFD488 Phalloidin XFD488 Same Structure to Alexa Fluor™ 488	499	520	73000	0.92¹	0.3	0.11
XFD488 acid Same Structure to Alexa Fluor™ 488 acid	499	520	71000	0.92¹	0.30	0.11
XFD488 tetrazine Same Structure to Alexa Fluor™ 488 tetrazine	499	520	71000	0.92¹	0.30	0.11
XFD488 aldehyde Same Structure to Alexa Fluor™ 488 aldehyde	499	520	71000	0.92¹	0.30	0.11
XFD350 TCO	343	441	19000	-	0.25	0.19
XFD405 TCO	401	421	35,000	-	0.23	0.70
XFD430 TCO	432	540	15,000	-	-	0.28
XFD514 TCO	518	543	80000	-	0.31	0.18
XFD546 TCO	561	572	112000	0.79¹	0.21	0.12
XFD532 TCO	534	553	81000	0.61¹	0.24	0.09
XFD568 TCO	579	603	91300	0.69¹	0.45	0.46
XFD594 TCO	590	618	90000	0.66¹	0.43	0.56
Cy3B TCO	560	571	120000¹	0.58¹	0.048	0.069
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References

View all 50 references: Citation Explorer

Detection of Homologous Blood Transfusion in Sport Doping by Flow Cytofluorimetry: State of the Art and New Approaches to Reduce the Risk of False-Negative Results.
Authors: Donati, Francesco and de la Torre, Xavier and Pagliarosi, Sarajane and Pirri, Daniela and Prevete, Giuliana and Botrè, Francesco
Journal: Frontiers in sports and active living (2022): 808449

A fluorescent aptasensor for the detection of Aflatoxin B1 by graphene oxide mediated quenching and release of fluorescence.
Authors: Setlem, Sai Keerthana and Mondal, Bhairab and Ramlal, Shylaja
Journal: Journal of microbiological methods (2022): 106414

Introducing Cysteines into Nanobodies for Site-Specific Labeling.
Authors: Hansen, Simon Boje and Andersen, Kasper Røjkjær
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 327-343

Dynamics of intracellular neonatal Fc receptor-ligand interactions in primary macrophages using biophysical fluorescence techniques.
Authors: Pannek, Andreas and Houghton, Fiona J and Verhagen, Anne M and Dower, Steven K and Hinde, Elizabeth and Gleeson, Paul A
Journal: Molecular biology of the cell (2022): ar6

Cholecystokinin-B Receptor-Targeted Nanoparticle for Imaging and Detection of Precancerous Lesions in the Pancreas.
Authors: Smith, Jill P and Cao, Hong and Edmondson, Elijah F and Dasa, Siva Sai Krishna and Stern, Stephan T
Journal: Biomolecules (2021)

Page updated on November 20, 2024

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Catalog Number	4601
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Physical properties

Molecular weight	945.16
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.3
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501