XFD514 TCO
XFD514, manufactured by AAT Bioquest, is a bright green-fluorescent dye that is structurally identical to Alexa Fluor™ 514 (ThermoFisher). With optimal excitation between 480-535 nm, it is ideally compatible with the 488 nm line of the Argon-Ion laser and the 532 nm line of the frequency-doubled Nd:YAG laser. XFD514 demonstrates excellent water solubility and remains stable over a wide pH range (pH 4–10), providing consistent and robust fluorescence signals. These properties make it highly suitable for advanced imaging techniques such as fluorescence microscopy and flow cytometry. XFD514 is a suitable replacement for rhodamine 6G.
XFD514 TCO is particularly useful for labeling tetrazine-modified biomolecules under copper-free conditions. It reacts with tetrazine-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD514 acid | 518 | 543 | 80000 | 0.31 | 0.18 |
XFD514 tyramide | 518 | 543 | 80000 | 0.31 | 0.18 |
XFD488 TCO | 499 | 520 | 73000 | 0.3 | 0.11 |
XFD350 TCO | 343 | 441 | 19000 | 0.25 | 0.19 |
XFD405 TCO | 401 | 421 | 35,000 | 0.23 | 0.70 |
XFD430 TCO | 432 | 540 | 15,000 | - | 0.28 |
XFD514 maleimide | 518 | 543 | 80000 | 0.31 | 0.18 |
XFD514 amine | 518 | 543 | 80000 | 0.31 | 0.18 |
XFD514 azide | 518 | 543 | 80000 | 0.31 | 0.18 |
Show More (7) |