XFD514 acid

• Ex/Em: 518/543 nm
• Extinction coefficient: 80,000 cm^-1M^-1
• Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
• Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

XFD514, manufactured by AAT Bioquest, is a green-fluorescent dye structurally identical to Alexa Fluor™ 514 (ThermoFisher). It is characterized by high photostability and exceptional brightness, making it highly suitable for advanced imaging and flow cytometry applications. The dye is water-soluble and remains pH-insensitive within a broad range (pH 4–10), ensuring consistent fluorescence performance across diverse experimental conditions. XFD514 offers robust and reliable labeling, enabling precise and reproducible results in fluorescence-based assays.

XFD514 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing XFD514 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.