XFD514 tyramide

• Ex/Em: 518/543 nm
• Extinction coefficient: 80,000 cm^-1M^-1
• Tyramide Signal Amplification (TSA): Facilitates ultrasensitive detection of low-abundance targets, ideal for IHC and ICC applications
• Bright & Stable XFD514 Dye: Delivers intense fluorescence with robust resistance to photobleaching and pH variations (4–10)

Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label translates ultrasensitive detection of low-abundance targets and the use of smaller amounts of antibodies and hybridization probes. In immunohistochemical applications, sensitivity enhancements derived from TSA method allow primary antibody dilutions to be increased to reduce nonspecific background signals, and can overcome weak immunolabeling caused by suboptimal fixation procedures or low levels of target expression. XFD 514 tyramide contains the Alexa Fluor® 514 fluorophore that can be readily detected with the standard rhodamine 6G filter set (Alexa Fluor® is the trademark of ThermoFisher). XFD 514 tyramide has intense green-yellow fluorescence color.