XFD350 acid *Same Structure to Alexa Fluor™ 350 acid*

• Ex/Em: 343/441 nm
• Extinction coefficient: 19,000 cm^-1M^-1
• Moderate Brightness: Optimized for high-abundance targets, providing clear and effective imaging in microscopy and flow cytometry
• pH Insensitivity: Maintains consistent fluorescence performance across a broad pH range (pH 4–11)
• High Contrast: Minimizes spectral overlap with common fluorophores like fluorescein and XFD488, enhancing multiplexing and signal clarity

XFD350, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor® 350 (ThermoFisher). This blue-fluorescent dye exhibits strong absorption and moderate photostability, with consistent fluorescence across a broad pH range (pH 4-11). XFD350 is effectively excited by the 350 nm line of a UV laser, and its moderate brightness and stability make it particularly advantageous for direct imaging of high-abundance targets in both microscopy and flow cytometry applications.

 XFD350 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing  XFD350 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.