XFD700 acid

Product key features

Ex/Em: 696/719 nm
Extinction coefficient: 205,000 cm^-1M^-1
Moderate Brightness: Ideal for high-abundance targets, preserving super-bright NIR dyes like APC for low-abundance labeling
Water Soluble: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies
Panel Compatibility: Fits seamlessly between APC and APC-iFluor® 780 in multicolor panels

Product description

XFD700 is the same molecule as the Alexa Fluor® 700 (Alexa Fluor® is the trademark of ThermoFisher). It is a bright and photostable near-IR dye that can be excited with a xenon-arc lamp, far-red diode lasers, or dye pumped lasers operating in the 675–700 nm range. It is used for stable signal generation in imaging and flow cytometry, and can be conjugated to antibodies, peptides, proteins, tracers, and amplification substrates. XFD700 has an absorption maximum at 702 nm and an emission maximum at 723 nm. It is compatible with all common excitation sources and instruments and can replace standard fluorophores such as Cy5.5 or IR680 for most applications.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD532 acid Same Structure to Alexa Fluor™ 532 acid	534	553	81000	0.61¹	0.24	0.09
XFD488 acid Same Structure to Alexa Fluor™ 488 acid	499	520	71000	0.92¹	0.30	0.11
XFD350 acid Same Structure to Alexa Fluor™ 350 acid	343	441	19000	-	0.25	0.19
XFD546 acid Same Structure to Alexa Fluor™ 546 acid	561	572	112000	0.79¹	0.21	0.12
XFD568 acid Same Structure to Alexa Fluor™ 568 acid	579	603	91300	0.69¹	0.45	0.46
XFD514 acid	518	543	80000	-	0.31	0.18
XFD555 acid	553	568	155000	0.1¹	0.08	0.08
XFD647 acid	650	671	270000	0.33¹	-	0.03
XFD750 acid	752	776	290000	0.12¹	-	0.04
XFD405 acid	401	421	35,000	-	0.23	0.70
XFD430 acid	432	540	15,000	-	-	0.28
XFD594 acid	590	618	90000	0.66¹	0.43	0.56
ICG acid	789	813	230000	-	-	0.076
Cy3NS acid CAS 1032678-01-5	555	569	150000¹	0.15¹	0.07	0.073
Cy5NS acid	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Cy7NS acid	756	779	250000	0.3	0.05	0.036
AMCA acid CAS#: 106562-32-7	346	434	19000	-	-	0.153
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] CAS 50402-56-7	336	455	5900	-	-	0.107
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]	336	455	5900	-	-	0.107
DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] CAS 6268-49-1	-	-	-	-	-	0.516
BCECF acid CAS#: 85138-49-4	504	527	90000	-	-	-
Cy3B acid	560	571	120000¹	0.58¹	0.048	0.069
QXY7 acid [equivalent to QSY-7 acid]	-	-	90000¹	-	-	0.22
OG514 acid [equivalent to Oregon Green® 514 carboxylic acid]	513	533	85000	-	-	0.19
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References

View all 40 references: Citation Explorer

Click-iT ® Plus OPP Alexa Fluor ® Protein Synthesis Assay in Embryonic Cells.
Authors: Li, Yan and Ji, Xu and Chang, Lu and Tang, Jianan and Hua, Min-Min and Liu, Jing and O'Neill, Christopher and Huang, Xuefeng and Jin, Xingliang
Journal: Bio-protocol (2022): e4441

Visualization of preimplantation uterine fluid absorption in mice using Alexa Fluor™ 488 hydrazide.
Authors: Li, Yuehuan and Martin, Taylor Elijah and Hancock, Jonathan Matthew and Li, Rong and Viswanathan, Suvitha and Lydon, John P and Zheng, Yi and Ye, Xiaoqin
Journal: Biology of reproduction (2022)

Alexa Fluor 488-conjugated cholera toxin subunit B optimally labels neurons 3-7 days after injection into the rat gastrocnemius muscle.
Authors: Cui, Jing-Jing and Wang, Jia and Xu, Dong-Sheng and Wu, Shuang and Guo, Ya-Ting and Su, Yu-Xin and Liu, Yi-Han and Wang, Yu-Qing and Jing, Xiang-Hong and Bai, Wan-Zhu
Journal: Neural regeneration research (2022): 2316-2320

Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series.
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021): 1546

Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase.
Authors: Ahishali, Bulent and Kaya, Mehmet
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 87-103

Page updated on November 21, 2024

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