XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*

• Ex/Em: 561/572 nm
• Extinction coefficient: 112,000 cm^-1M^-1
• High Quantum Yield & Stability: Exhibits intense fluorescence with resilience to photobleaching, thermal fluctuations, and pH variations from 4 to 10
• Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies
• Enhanced Sensitivity: Detects low-abundance targets with precision, improving quantitative fluorescence assay performance

XFD546, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 546 (ThermoFisher). It exhibits bright orange fluorescence and is readily excited by laser lines at 488 nm or 532 nm, making it highly suitable for applications such as fluorescence microscopy and flow cytometry. XFD546 demonstrates excellent aqueous solubility and pH-insensitivity over a broad range (pH 4–10), ensuring stable signal generation under varying experimental conditions. Additionally, the dye enables high molar ratio conjugation to proteins with minimal fluorescence quenching, facilitating the generation of brighter conjugates for enhanced detection sensitivity. With its high fluorescence quantum yield and superior photostability, XFD546 is particularly advantageous for detecting low-abundance biological targets, providing researchers with improved sensitivity and precision in quantitative fluorescence-based assays.

XFD546 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing XFD546 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.