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XFD488 acid *Same Structure to Alexa Fluor™ 488 acid*
Product key features
- Ex/Em: 499/520 nm
- Extinction coefficient: 71,000 cm-1M-1
- Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
- Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications
Product description
XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). XFD488 acid has the same molecule as Alexa Fluor® 488 acid. It is a bright yellow-fluorescent dye optimal for use with the 488 nm Argon laser line. XFD488 dye is water soluble and pH-insensitive from pH 4 to pH 10. XFD488 acid is the most economic amine-reactive form for conjugating this dye to a peptide.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| XFD350 acid *Same Structure to Alexa Fluor™ 350 acid* | 343 | 441 | 19000 | - | 0.25 | 0.19 |
| XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| XFD488 Hydroxylamine *Same Structure to Alexa Fluor™ 488 Hydroxylamine* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| XFD488 TCO | 499 | 520 | 73000 | 0.921 | 0.3 | 0.11 |
| XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| XFD514 acid | 518 | 543 | 80000 | - | 0.31 | 0.18 |
| XFD532 acid *Same Structure to Alexa Fluor™ 532 acid* | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
| XFD546 acid *Same Structure to Alexa Fluor™ 546 acid* | 561 | 572 | 112000 | 0.791 | 0.21 | 0.12 |
| XFD555 acid | 553 | 568 | 155000 | 0.11 | 0.08 | 0.08 |
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Citations
View all 1 citations: Citation Explorer
Characterization of Biomolecular Interactions by Biophysical Methods.
Authors: Demeler, Aysha K and Bosco, James and Sydor, Matthew J and Nemetchek, Michelle and Mortezazadeh, Saeed and Kerr, Liam and Bird, Sophia and Gabdullina, Roza and Yates-Hansen, Cindee and McClelland, Levi J and others,
Journal: Analytical Biochemistry (2025): 115859
Authors: Demeler, Aysha K and Bosco, James and Sydor, Matthew J and Nemetchek, Michelle and Mortezazadeh, Saeed and Kerr, Liam and Bird, Sophia and Gabdullina, Roza and Yates-Hansen, Cindee and McClelland, Levi J and others,
Journal: Analytical Biochemistry (2025): 115859
References
View all 39 references: Citation Explorer
Development of new hCaM-Alexa Fluor(R) biosensors for a wide range of ligands
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep
Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)
Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601
In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12
Page updated on April 15, 2025
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