XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*

• Ex/Em: 499/520 nm
• Extinction coefficient: 71,000 cm^-1M^-1
• Reactive Group: Aldehyde
• Versatile Conjugation: Reacts with amines, hydrazides, or hydroxylamines from pH 5-9, and can target N-terminal amine groups at acidic pH
• Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4-10
• Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). XFD488 aldehyde contains the fluorophore of XFD488. It is a reactive green fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated XFD488 dye can be easily detected by a common fluorescence instrument under FITC channel.