XFD488 tetrazine Same Structure to Alexa Fluor™ 488 tetrazine

Product key features

Ex/Em: 499/520 nm
Extinction coefficient: 71,000 cm^-1M^-1
Reactive Group: Tetrazine
Click Chemistry Conjugation: Provides rapid, copper-free labeling of TCO-modified biomolecules
Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

Product description

XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). XFD488 is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line. It is used for stable signal generation in imaging and flow cytometry. XFD488 tetrazine demonstrates exceptionally fast reaction with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any biorthogonal reaction. It provides a convenient tool for making biological conjugates with TCO-modified substrates. The resulted conjugates provide the bright, green fluorescence of XFD488.

Spectrum

Open in Advanced Spectrum Viewer

Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD488 azide Same Structure to Alexa Fluor™ 488 azide	499	520	71000	0.92¹	0.30	0.11
XFD488 alkyne Same Structure to Alexa Fluor™ 488 alkyne	499	520	71000	0.92¹	0.30	0.11
XFD488 Hydroxylamine Same Structure to Alexa Fluor™ 488 Hydroxylamine	499	520	71000	0.92¹	0.30	0.11
XFD488 amine Same Structure to Alexa Fluor™ 488 amine	499	520	71000	0.92¹	0.30	0.11
XFD488 Phalloidin XFD488 Same Structure to Alexa Fluor™ 488	499	520	73000	0.92¹	0.3	0.11
XFD488 acid Same Structure to Alexa Fluor™ 488 acid	499	520	71000	0.92¹	0.30	0.11
XFD488 aldehyde Same Structure to Alexa Fluor™ 488 aldehyde	499	520	71000	0.92¹	0.30	0.11
XFD488 TCO	499	520	73000	0.92¹	0.3	0.11
XFD350 tetrazine	343	441	19000	-	0.25	0.19
XFD405 Tetrazine	401	421	35,000	-	0.23	0.70
XFD430 Tetrazine	432	540	15,000	-	-	0.28
XFD514 Tetrazine	518	543	80000	-	0.31	0.18
XFD546 Tetrazine	561	572	112000	0.79¹	0.21	0.12
XFD532 Tetrazine	534	553	81000	0.61¹	0.24	0.09
XFD568 Tetrazine	579	603	91300	0.69¹	0.45	0.46
XFD594 Tetrazine	590	618	90000	0.66¹	0.43	0.56
XFD610 tetrazine	611	629	144,000	-	0.43	0.44
XFD633 Tetrazine	632	650	159,000	-	-	0.55
XFD660 Tetrazine	663	691	132000	-	0.00	0.10
Cy3 tetrazine	555	569	150000¹	0.15¹	0.07	0.073
Cy5 tetrazine	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Cy3B Tetrazine	560	571	120000¹	0.58¹	0.048	0.069
Cy7 tetrazine	756	779	250000	0.3	0.05	0.036
Show More (14)

References

View all 37 references: Citation Explorer

Nanobody click chemistry for convenient site-specific fluorescent labelling, single step immunocytochemistry and delivery into living cells by photoporation and live cell imaging.
Authors: Hebbrecht, Tim and Liu, Jing and Zwaenepoel, Olivier and Boddin, Gaëlle and Van Leene, Chloé and Decoene, Klaas and Madder, Annemieke and Braeckmans, Kevin and Gettemans, Jan
Journal: New biotechnology (2020): 33-43

A novel tracer for in vivo optical imaging of fatty acid metabolism in the heart and brown adipose tissue.
Authors: Panagia, Marcello and Yang, Jing and Gale, Eric and Wang, Huan and Luptak, Ivan and Chen, Howard H and Patel, Dakshesh and Croteau, Dominique and Pimentel, David Richard and Bachschmid, Markus Michael and Colucci, Wilson S and Ran, Chongzhao and Sosnovik, David E
Journal: Scientific reports (2020): 11209

Tylophorine reduces protein biosynthesis and rapidly decreases cyclin D1, inhibiting vascular smooth muscle cell proliferation in vitro and in organ culture.
Authors: Joa, Helge and Blažević, Tina and Grojer, Christoph and Zeller, Iris and Heiss, Elke H and Atanasov, Atanas G and Feldler, Ines and Gruzdaitis, Päivi and Czaloun, Christa and Proksch, Peter and Messner, Barbara and Bernhard, David and Dirsch, Verena M
Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (2019): 152938

PET imaging of EGFR expression using an 18F-labeled RNA aptamer.
Authors: Cheng, Siyuan and Jacobson, Orit and Zhu, Guizhi and Chen, Zhen and Liang, Steve H and Tian, Rui and Yang, Zhen and Niu, Gang and Zhu, Xiaohua and Chen, Xiaoyuan
Journal: European journal of nuclear medicine and molecular imaging (2019): 948-956

Long-term live cells observation of internalized fluorescent Fe@C nanoparticles in constant magnetic field.
Authors: Garanina, Anastasiia and Kireev, Igor and Zhironkina, Oxana and Strelkova, Olga and Shakhov, Anton and Alieva, Irina and Davydov, Valery and Murugesan, Sankaran and Khabashesku, Valery and Majouga, Alexander and Agafonov, Viatcheslav and Uzbekov, Rustem
Journal: Journal of nanobiotechnology (2019): 27

Page updated on December 17, 2024

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Catalog Number	4600
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Physical properties

Molecular weight	717.68
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.30
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501