XFD635 Tetrazine

• Ex/Em: 633/647 nm
• Extinction Coefficient: 140,000 cm^-1M^-1
• Reactive Group: Tetrazine
• Click Chemistry Conjugation: Provides rapid, copper-free labeling of TCO-modified biomolecules
• Advanced Dye Properties: Excellent water solubility and structural match to Alexa Fluor™ 635
• Robust Signal Performance: Maintains high intensity and pH independence (pH 4-11) with exceptional photostability

XFD635, manufactured by AAT Bioquest, is a bright far red-fluorescent dye structurally identical to Alexa Fluor™ 635 (ThermoFisher). It is characterized by its intense brightness, high fluorescence quantum yield and photostability. The dye demonstrates excellent solubility in aqueous solutions and retains pH-independent fluorescence over a broad range (pH 4–11), ensuring consistent performance across diverse experimental conditions. Optimally excited by the 633 nm emission line of He-Ne lasers or the 635 nm diode laser, XFD635 is particularly well-suited for flow cytometry. Its robust and uniform labeling properties yield high signal intensity and reproducibility, making it an ideal choice for advanced fluorescence imaging, flow cytometry, and various fluorescence-based analytical techniques.

XFD635 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.