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• Ex/Em: 633/647 nm
• Extinction Coefficient: 140,000 cm^-1M^-1
• Reactive Group: Amine
• Versatile Conjugation: Efficiently tags carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
• Advanced Dye Properties: Excellent water solubility and structural match to Alexa Fluor™ 635
• Robust Signal Performance: Maintains high intensity and pH independence (pH 4-11) with exceptional photostability

XFD635, manufactured by AAT Bioquest, is a bright far red-fluorescent dye structurally identical to Alexa Fluor™ 635 (ThermoFisher). It is characterized by its intense brightness, high fluorescence quantum yield and photostability. The dye demonstrates excellent solubility in aqueous solutions and retains pH-independent fluorescence over a broad range (pH 4–11), ensuring consistent performance across diverse experimental conditions. Optimally excited by the 633 nm emission line of He-Ne lasers or the 635 nm diode laser, XFD635 is particularly well-suited for flow cytometry. Its robust and uniform labeling properties yield high signal intensity and reproducibility, making it an ideal choice for advanced fluorescence imaging, flow cytometry, and various fluorescence-based analytical techniques.

XFD635 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.