XFD790 amine

• Ex/Em: 782/805 nm
• Extinction coefficient: 260,000 cm^-1M^-1
• Reactive Group: Amine
• Easy Conjugation: Efficiently tags carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
• Water Soluble: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies
• Reduced Autofluorescence: Long-wavelength emission minimizes background interference, enhancing signal-to-noise ratios in complex biological matrices.
• Panel Optimization: Well-separated from far-red fluorophores such as iFluor® 647 and allophycocyanin (APC), facilitating precise multicolor fluorescence detection.

XFD790, manufactured by AAT Bioquest, is a highly efficient near-infrared fluorescent dye that is structurally identical to Alexa Fluor™ 790 (ThermoFisher). Spectrally analogous to indocyanine green (ICG) and IRDye™ 800, XFD790 demonstrates exceptional aqueous solubility and sustained fluorescence stability over a broad pH range (pH 4–10), ensuring consistent and reproducible performance across diverse experimental conditions. Its long-wavelength emission effectively mitigates background autofluorescence, thereby enhancing signal-to-noise ratios in complex biological matrices, including tissue samples. As the longest-wavelength fluorophore in the XFD series, XFD790 offers superior spectral separation from widely used far-red fluorophores such as iFluor® 647, XFD647, and allophycocyanin (APC), facilitating precise multicolor fluorescence analyses. Furthermore, its optical properties make it an excellent candidate for small animal in vivo imaging (SAIVI) and two-color western blot applications using the LI-COR™ Odyssey™ infrared imaging system.

XFD790 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.