XFD700 amine

• Ex/Em: 696/719 nm
• Extinction coefficient: 192,000 cm^-1M^-1
• Reactive Group: Amine
• Easy Conjugation: Efficiently tags carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
• Moderate Brightness: Suitable for high-abundance targets, preserving brighter dyes for low-abundance detection.
• Water Soluble: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies
• Panel Optimization: Enhances spectral resolution between APC and APC-iFluor® 780 in multicolor assays.

XFD700, manufactured by AAT Bioquest, is a near-infrared fluorescent dye structurally identical to Alexa Fluor™ 700 (ThermoFisher). It is optimally excited by 633–640 nm laser lines and offers a relatively low fluorescence intensity, making it particularly well-suited for direct imaging of high-abundance targets in both microscopy and flow cytometry. This allows researchers to allocate brighter dyes for detecting lower-abundance antigens, improving overall panel design. XFD700 exhibits excellent aqueous solubility and maintains consistent fluorescence stability across a broad pH range (pH 4–10), ensuring robust and reproducible performance under diverse experimental conditions. Its long-wavelength emission effectively minimizes background autofluorescence, leading to enhanced signal-to-noise ratios, especially in complex biological samples such as tissues. In multicolor flow cytometry panels, XFD700 serves as an ideal option between APC and APC-iFluor® 780, enabling better resolution in complex assays.

XFD700 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.