XFD647 amine

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Physical properties

Molecular weight	1129.14
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.03
Correction Factor (565 nm)	0.1109
Extinction coefficient (cm ^-1 M ^-1)	270000
Excitation (nm)	650
Emission (nm)	671
Quantum yield	0.33¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Direct upgrades

Alternative formats

Overview SDS Protocol

Upgrade: iFluor® 647 amine

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD647 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	88.563 µL	442.815 µL	885.63 µL	4.428 mL	8.856 mL
5 mM	17.713 µL	88.563 µL	177.126 µL	885.63 µL	1.771 mL
10 mM	8.856 µL	44.281 µL	88.563 µL	442.815 µL	885.63 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.03
Correction Factor (565 nm)	0.1109
Extinction coefficient (cm ^-1 M ^-1)	270000
Excitation (nm)	650
Emission (nm)	671
Quantum yield	0.33¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD488 amine Same Structure to Alexa Fluor™ 488 amine	499	520	71000	0.92¹	0.30	0.11
XFD594 amine Same Structure to Alexa Fluor™ 594 amine	590	618	90000	0.66¹	0.43	0.56
XFD532 amine	534	553	81000	0.61¹	0.24	0.09
XFD555 amine	553	568	155000	0.1¹	0.08	0.08
XFD568 amine	579	603	88000	0.69¹	0.45	0.46
XFD750 amine	752	776	290000	0.12¹	-	0.04
XFD647 Phalloidin equivalent to Alexa Fluor® 647 phalloidin	650	671	239000	0.33¹	0.00	0.03
ICG amine	789	813	230000	-	-	0.076

Images

Figure 1. XFD647 amine is the same molecule to the Alexa Fluor® 647 amine. It is a cyanine dye with bright red-fluorescence optimal for labeling antibodies.

References

View all 50 references: Citation Explorer

Flow Cytometric Identification and Enumeration of Monocyte Subsets in Bovine and Water Buffalo Peripheral Blood.
Authors: Grandoni, Francesco and Fraboni, Daniela and Canonico, Barbara and Papa, Stefano and Buccisano, Francesco and Schuberth, Hans-Joachim and Hussen, Jamal
Journal: Current protocols (2023): e676

Diyne inactivators and activity-based fluorescent labeling of phenol hydroxylase in Pseudomonas sp. CF600.
Authors: Oyarzun Mejia, Alejandra P and Hyman, Michael R
Journal: FEMS microbiology letters (2023)

In Vivo Evaluation of Sgc8-c Aptamer as a Molecular Imaging Probe for Colon Cancer in a Mouse Xenograft Model.
Authors: Arévalo, Ana Paula and Castelli, Romina and Ibarra, Manuel and Crispo, Martina and Calzada, Victoria
Journal: International journal of molecular sciences (2022)

A 5-color flow cytometry panel to assess plasma membrane integrity, acrosomal status, membrane lipid organization and mitochondrial activity of boar and stallion spermatozoa following liquid semen storage.
Authors: Quirino, Monike and Jakop, Ulrike and Mellagi, Ana Paula Gonçalves and Bortolozzo, Fernando Pandolfo and Jung, Markus and Schulze, Martin
Journal: Animal reproduction science (2022): 107076

Determination of FRET orientation factor between artificial fluorophore and photosynthetic light-harvesting 2 complex (LH2).
Authors: Fujimoto, Kazuhiro J and Miyashita, Tomoya and Dewa, Takehisa and Yanai, Takeshi
Journal: Scientific reports (2022): 15091

UCsim2: two-dimensionally structured illumination microscopy using UC2.
Authors: Wang, Haoran and Lachmann, René and Marsikova, Barbora and Heintzmann, Rainer and Diederich, Benedict
Journal: Philosophical transactions. Series A, Mathematical, physical, and engineering sciences (2022): 20200148

Highly sensitive fluorescent detection of EDIL3 overexpressed exosomes for the diagnosis of triple-negative breast cancer.
Authors: Wei, Ying-Xiang and Han, Jia-Hao and Shen, Hui-Ming and Wang, Ying-Yan and Qi, Min and Wang, Ling and Li, Jia
Journal: Nanotechnology (2022)

[The Establishment and Application of Flow Cytometry on the Detection of Jka Antigen in Kidd Blood Group].
Authors: Zhang, Hao and Liang, Shuang and Li, Le-Bing and Su, Yu-Qing
Journal: Zhongguo shi yan xue ye xue za zhi (2022): 250-255

Synthesis of a theranostic platform based on fibrous silica nanoparticles for the enhanced treatment of triple-negative breast cancer promoted by a combination of chemotherapeutic agents.
Authors: Ovejero-Paredes, Karina and Díaz-García, Diana and Mena-Palomo, Irene and Marciello, Marzia and Lozano-Chamizo, Laura and Morato, Yurena Luengo and Prashar, Sanjiv and Gómez-Ruiz, Santiago and Filice, Marco
Journal: Biomaterials advances (2022): 212823

A Fluorescent Sensor for Quantitative Super-Resolution Imaging of Amyloid Fibril Assembly.
Authors: Kaur, Amandeep and Adair, Liam D and Ball, Sarah R and New, Elizabeth J and Sunde, Margaret
Journal: Angewandte Chemie (International ed. in English) (2022): e202112832