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XFD647 Alkyne
Product key features
- Ex/Em: 650/671 nm
- Extinction coefficient: 270,000 cm-1M-1
- Reactive Group: Alkyne
- Efficient Conjugation: Click chemistry labeling of azides on peptides, antibodies, and other biomolecules
- Bright & Stable: Delivers intense fluorescence with resilience to photobleaching and pH variations from 4 to 10
- Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies
Product description
XFD647 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 647 (Alexa Fluor® is the trademark of ThermoFisher). It is a bright deep red fluorescent dye. XFD647 dye is water soluble and pH-insensitive from pH 4 to pH 10. XFD647 alkyne is used for conjugation with a biological molecule that contains an azide via the click chemistry. It provides a convenient way to install the fluorophore of Alexa Fluor® 647 into a biological molecule that lacks alkyne and azide groups.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne* | 499 | 520 | 71000 | 0.11 |
| XFD647 Phalloidin *equivalent to Alexa Fluor® 647 phalloidin* | 650 | 671 | 239000 | 0.03 |
| XFD350 alkyne | 343 | 441 | 19000 | 0.19 |
| XFD405 alkyne | 401 | 421 | 35,000 | 0.70 |
| XFD430 alkyne | 432 | 540 | 15,000 | 0.28 |
| XFD514 alkyne | 518 | 543 | 80000 | 0.18 |
| XFD546 alkyne | 561 | 572 | 112000 | 0.12 |
| XFD532 alkyne | 534 | 553 | 81000 | 0.09 |
| XFD568 alkyne | 579 | 603 | 91300 | 0.46 |
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References
View all 42 references: Citation Explorer
Protocol for visualizing newly synthesized proteins in primary mouse hepatocytes.
Authors: Shen, Yuqian and Liu, Wenhua and Zuo, Jian and Han, Junhai and Zhang, Zi Chao
Journal: STAR protocols (2021): 100616
Authors: Shen, Yuqian and Liu, Wenhua and Zuo, Jian and Han, Junhai and Zhang, Zi Chao
Journal: STAR protocols (2021): 100616
EdU Incorporation To Assess Cell Proliferation and Drug Susceptibility in Naegleria fowleri.
Authors: Troth, Emma V and Kyle, Dennis E
Journal: Antimicrobial agents and chemotherapy (2021): e0001721
Authors: Troth, Emma V and Kyle, Dennis E
Journal: Antimicrobial agents and chemotherapy (2021): e0001721
Azide-Functionalized Naphthoxyloside as a Tool for Glycosaminoglycan Investigations.
Authors: Willén, Daniel and Mastio, Roberto and Söderlund, Zackarias and Manner, Sophie and Westergren-Thorsson, Gunilla and Tykesson, Emil and Ellervik, Ulf
Journal: Bioconjugate chemistry (2021): 2507-2515
Authors: Willén, Daniel and Mastio, Roberto and Söderlund, Zackarias and Manner, Sophie and Westergren-Thorsson, Gunilla and Tykesson, Emil and Ellervik, Ulf
Journal: Bioconjugate chemistry (2021): 2507-2515
SMALL MOLECULE IMAGING AGENT FOR MUTANT KRAS G12C.
Authors: Koch, Peter D and Quintana, Jeremy and Ahmed, Maaz and Kohler, Rainer H and Weissleder, Ralph
Journal: Advanced therapeutics (2021)
Authors: Koch, Peter D and Quintana, Jeremy and Ahmed, Maaz and Kohler, Rainer H and Weissleder, Ralph
Journal: Advanced therapeutics (2021)
Clickable Galactose Analogues for Imaging Glycans in Developing Zebrafish.
Authors: Daughtry, Jessica L and Cao, Wendy and Ye, Johnny and Baskin, Jeremy M
Journal: ACS chemical biology (2020): 318-324
Authors: Daughtry, Jessica L and Cao, Wendy and Ye, Johnny and Baskin, Jeremy M
Journal: ACS chemical biology (2020): 318-324
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