XFD647 Azide

• Ex/Em: 650/671 nm
• Extinction coefficient: 270,000 cm^-1M^-1
• Reactive Group: Azide
• Click Chemistry Conjugation: Efficiently and rapidly labels terminal alkynes via CuAAC and copper-free SPAAC
• Bright & Stable: Delivers intense fluorescence with resilience to photobleaching and pH variations from 4 to 10
• Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies

XFD647, manufactured by AAT Bioquest, is a bright far-red flurosecent dye structurally identical to Alexa Fluor™ 647 (ThermoFisher). It is efficiently excited by the 594 nm or 633 nm laser lines and is compatible with RFP filters like Cy5, making it well-suited for applications such as fluorescence microscopy and flow cytometry. XFD647 exhibits excellent aqueous solubility and pH stability across a wide range (pH 4–10), ensuring robust signal reproducibility under diverse experimental conditions. The dye enables high-degree conjugation to biomolecules with minimal self-quenching, resulting in enhanced fluorescence intensity and signal stability. Additionally, its high fluorescence quantum yield and superior photostability facilitate the sensitive detection of low-abundance targets, enhancing precision and sensitivity in quantitative fluorescence-based assays.

The azide derivative of XFD647 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.