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AAT Bioquest

XFD647 Azide

Product key features

  • Ex/Em: 650/671 nm
  • Extinction coefficient: 270,000 cm-1M-1
  • Reactive Group: Azide
  • Click Chemistry Conjugation: Efficiently and rapidly labels terminal alkynes via CuAAC and copper-free SPAAC
  • Bright & Stable: Delivers intense fluorescence with resilience to photobleaching and pH variations from 4 to 10
  • Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies

Product description

XFD647 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 647 (Alexa Fluor® is the trademark of ThermoFisher). It is a bright deep red fluorescent dye. XFD647 dye is water soluble and pH-insensitive from pH 4 to pH 10. XFD647 azide is used for conjugation with a biological molecule that contains an alkyne via the click chemistry. It provides a convenient way to install the fluorophore of Alexa Fluor® 647 into a biological molecule that lacks alkyne and azide groups.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide*499520710000.11
XFD647 Phalloidin *equivalent to Alexa Fluor® 647 phalloidin*6506712390000.03
XFD350 azide343441190000.19
XFD405 azide40142135,0000.70
XFD430 azide43254015,0000.28
XFD514 azide518543800000.18
XFD546 azide5615721120000.12
XFD532 azide534553810000.09
XFD568 azide579603913000.46
XFD594 azide590618900000.56
XFD610 azide611629144,0000.44
XFD633 azide632650159,0000.55
XFD660 azide6636911320000.10
AMCA Azide346434190000.153
ICG azide7898132300000.076
Cy3B azide56057112000010.069
Show More (7)

References

View all 9 references: Citation Explorer
Azide-Functionalized Naphthoxyloside as a Tool for Glycosaminoglycan Investigations.
Authors: Willén, Daniel and Mastio, Roberto and Söderlund, Zackarias and Manner, Sophie and Westergren-Thorsson, Gunilla and Tykesson, Emil and Ellervik, Ulf
Journal: Bioconjugate chemistry (2021): 2507-2515
SMALL MOLECULE IMAGING AGENT FOR MUTANT KRAS G12C.
Authors: Koch, Peter D and Quintana, Jeremy and Ahmed, Maaz and Kohler, Rainer H and Weissleder, Ralph
Journal: Advanced therapeutics (2021)
Bioorthogonal Labeling of Human Prostate Cancer Tissue Slice Cultures for Glycoproteomics.
Authors: Spiciarich, David R and Nolley, Rosalie and Maund, Sophia L and Purcell, Sean C and Herschel, Jason and Iavarone, Anthony T and Peehl, Donna M and Bertozzi, Carolyn R
Journal: Angewandte Chemie (International ed. in English) (2017): 8992-8997
Activity-Based Protein Profiling of Ammonia Monooxygenase in Nitrosomonas europaea.
Authors: Bennett, Kristen and Sadler, Natalie C and Wright, Aaron T and Yeager, Chris and Hyman, Michael R
Journal: Applied and environmental microbiology (2016): 2270-2279
Plasmonic nanodiamonds: targeted core-shell type nanoparticles for cancer cell thermoablation.
Authors: Rehor, Ivan and Lee, Karin L and Chen, Kevin and Hajek, Miroslav and Havlik, Jan and Lokajova, Jana and Masat, Milan and Slegerova, Jitka and Shukla, Sourabh and Heidari, Hamed and Bals, Sara and Steinmetz, Nicole F and Cigler, Petr
Journal: Advanced healthcare materials (2015): 460-8
Page updated on December 17, 2024

Ordering information

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Catalog Number1896
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Physical properties

Molecular weight

1041.11

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.03

Correction Factor (565 nm)

0.1109

Extinction coefficient (cm -1 M -1)

270000

Excitation (nm)

650

Emission (nm)

671

Quantum yield

0.331

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501