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AAT Bioquest

XFD647 Azide

Product key features

  • Ex/Em: 650/671 nm
  • Extinction coefficient: 270,000 cm-1M-1
  • Reactive Group: Azide
  • Click Chemistry Conjugation: Efficiently and rapidly labels terminal alkynes via CuAAC and copper-free SPAAC
  • Bright & Stable: Delivers intense fluorescence with resilience to photobleaching and pH variations from 4 to 10
  • Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies

Product description

XFD647 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 647 (Alexa Fluor® is the trademark of ThermoFisher). It is a bright deep red fluorescent dye. XFD647 dye is water soluble and pH-insensitive from pH 4 to pH 10. XFD647 azide is used for conjugation with a biological molecule that contains an alkyne via the click chemistry. It provides a convenient way to install the fluorophore of Alexa Fluor® 647 into a biological molecule that lacks alkyne and azide groups.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide*499520710000.11
XFD647 Phalloidin *equivalent to Alexa Fluor® 647 phalloidin*6506712390000.03
XFD350 azide343441190000.19
XFD405 azide40142135,0000.70
XFD430 azide43254015,0000.28
XFD514 azide518543800000.18
XFD546 azide5615721120000.12
XFD532 azide534553810000.09
XFD568 azide579603913000.46
XFD594 azide590618900000.56
AMCA Azide346434190000.153
ICG azide7898132300000.076
Cy3B azide56057112000010.069
Show More (4)

References

View all 9 references: Citation Explorer
Azide-Functionalized Naphthoxyloside as a Tool for Glycosaminoglycan Investigations.
Authors: Willén, Daniel and Mastio, Roberto and Söderlund, Zackarias and Manner, Sophie and Westergren-Thorsson, Gunilla and Tykesson, Emil and Ellervik, Ulf
Journal: Bioconjugate chemistry (2021): 2507-2515
SMALL MOLECULE IMAGING AGENT FOR MUTANT KRAS G12C.
Authors: Koch, Peter D and Quintana, Jeremy and Ahmed, Maaz and Kohler, Rainer H and Weissleder, Ralph
Journal: Advanced therapeutics (2021)
Bioorthogonal Labeling of Human Prostate Cancer Tissue Slice Cultures for Glycoproteomics.
Authors: Spiciarich, David R and Nolley, Rosalie and Maund, Sophia L and Purcell, Sean C and Herschel, Jason and Iavarone, Anthony T and Peehl, Donna M and Bertozzi, Carolyn R
Journal: Angewandte Chemie (International ed. in English) (2017): 8992-8997
Activity-Based Protein Profiling of Ammonia Monooxygenase in Nitrosomonas europaea.
Authors: Bennett, Kristen and Sadler, Natalie C and Wright, Aaron T and Yeager, Chris and Hyman, Michael R
Journal: Applied and environmental microbiology (2016): 2270-2279
Plasmonic nanodiamonds: targeted core-shell type nanoparticles for cancer cell thermoablation.
Authors: Rehor, Ivan and Lee, Karin L and Chen, Kevin and Hajek, Miroslav and Havlik, Jan and Lokajova, Jana and Masat, Milan and Slegerova, Jitka and Shukla, Sourabh and Heidari, Hamed and Bals, Sara and Steinmetz, Nicole F and Cigler, Petr
Journal: Advanced healthcare materials (2015): 460-8
Page updated on November 20, 2024

Ordering information

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Catalog Number1896
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Physical properties

Molecular weight

1041.11

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.03

Correction Factor (565 nm)

0.1109

Extinction coefficient (cm -1 M -1)

270000

Excitation (nm)

650

Emission (nm)

671

Quantum yield

0.331

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501