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AAT Bioquest

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Product key features

  • Ex/Em: 681/704 nm
  • Extinction coefficient:184,000 cm-1M-1
  • Superior Fluorescence Performance: High quantum yield, exceptional photostability, and pH-independent fluorescence across pH 4–11.
  • Reactive Group: Tetrazine
  • Click Chemistry Conjugation: Provides rapid, copper-free labeling of TCO-modified biomolecules
  • High Water-Solubility: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

Product description

XFD680, manufactured by AAT Bioquest, is a bright near-infrared fluorescent dye structurally equivalent to Alexa Fluor™ 680 (ThermoFisher). It exhibits a high fluorescence quantum yield, excellent photostability, and superior aqueous solubility, ensuring consistent and reliable performance in various applications. Its pH-independent fluorescence across a broad range (pH 4–11) allows it to maintain stability under diverse experimental conditions. The dye also enables high molar ratio protein conjugation with minimal self-quenching, producing brighter conjugates and enhancing detection sensitivity. Its long-wavelength emission minimizes interference from autofluorescent background signals, enabling accurate detection in complex biological systems.

XFD680 is optimized for red laser excitation and is compatible with flow cytometers equipped with spectral detection systems. It delivers robust and uniform labeling, with high signal intensity and reproducibility, making it ideal for fluorescence imaging, flow cytometry, and other analytical techniques. XFD680 is also widely utilized in advanced applications such as multiplexed western blot detection and stochastic optical reconstruction microscopy (STORM), where its superior photophysical properties enhance resolution and sensitivity.

XFD680 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*499520710000.9210.300.11
XFD350 tetrazine34344119000-0.250.19
XFD405 Tetrazine40142135,000-0.230.70
XFD430 Tetrazine43254015,000--0.28
XFD514 Tetrazine51854380000-0.310.18
XFD546 Tetrazine5615721120000.7910.210.12
XFD532 Tetrazine534553810000.6110.240.09
XFD568 Tetrazine579603913000.6910.450.46
XFD594 Tetrazine590618900000.6610.430.56
XFD610 tetrazine611629144,000-0.430.44
XFD633 Tetrazine632650159,000--0.55
XFD660 Tetrazine663691132000-0.000.10
XFD680 acid6817041840000.3610.000.05
XFD680 Maleimide6817041840000.3610.000.05
XFD680 amine6817041840000.3610.000.05
XFD680 azide6817041840000.3610.000.05
XFD680 alkyne6817041840000.3610.000.05
XFD680 TCO6817041840000.3610.000.05
Cy3 tetrazine55556915000010.1510.070.073
Cy5 tetrazine65167025000010.271, 0.420.020.03
Cy3B Tetrazine56057112000010.5810.0480.069
Cy7 tetrazine7567792500000.30.050.036
Show More (13)
Page updated on January 20, 2025

Ordering information

Price
Unit size
Catalog Number70108
Quantity
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
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Physical properties

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.00

Correction Factor (280 nm)

0.05

Extinction coefficient (cm -1 M -1)

184000

Excitation (nm)

681

Emission (nm)

704

Quantum yield

0.361

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501