XFD532 Tetrazine

• Ex/Em: 534/553 nm
• Extinction coefficient: 81,000 cm^-1M^-1
• Reactive Group: Tetrazine
• Click Chemistry Conjugation: Provides rapid, copper-free labeling of TCO-modified biomolecules
• Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
• Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell studies

XFD532, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 532 (ThermoFisher). It is a bright yellow-fluorescent dye with an excitation optimized for use with the 532 nm line of the frequency-doubled Nd:YAG laser. XFD532 demonstrates good aqueous solubility and pH-insensitivity over a broad pH range (pH 4–10), ensuring stable fluorescence generation under varying experimental conditions. This dye is particularly suited for multicolor fluorescence microscopy and flow cytometry, as well as advanced applications in super-resolution imaging techniques such as dSTORM.

XFD532 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.