XFD532 Tetrazine
XFD532, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 532 (ThermoFisher). It is a bright yellow-fluorescent dye with an excitation optimized for use with the 532 nm line of the frequency-doubled Nd:YAG laser. XFD532 demonstrates good aqueous solubility and pH-insensitivity over a broad pH range (pH 4–10), ensuring stable fluorescence generation under varying experimental conditions. This dye is particularly suited for multicolor fluorescence microscopy and flow cytometry, as well as advanced applications in super-resolution imaging techniques such as dSTORM.
XFD532 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid* | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD532 tyramide | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
XFD532 amine | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
XFD350 tetrazine | 343 | 441 | 19000 | - | 0.25 | 0.19 |
XFD405 Tetrazine | 401 | 421 | 35,000 | - | 0.23 | 0.70 |
XFD430 Tetrazine | 432 | 540 | 15,000 | - | - | 0.28 |
XFD514 Tetrazine | 518 | 543 | 80000 | - | 0.31 | 0.18 |
XFD546 Tetrazine | 561 | 572 | 112000 | 0.791 | 0.21 | 0.12 |
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