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Cy3 tetrazine
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This inverse electron demand cycloaddition reaction has gained popularity due to the potential for extremely fast cycloaddition kinetics with TCO as the dienophile. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this poweful click reaction. Cy3-tetrazine has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. It is widely used for labeling peptides, proteins and oligos etc. Cy3 dyes have enhanced fluorescence upon binding to proteins.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3 tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 110.124 µL | 550.618 µL | 1.101 mL | 5.506 mL | 11.012 mL |
| 5 mM | 22.025 µL | 110.124 µL | 220.247 µL | 1.101 mL | 2.202 mL |
| 10 mM | 11.012 µL | 55.062 µL | 110.124 µL | 550.618 µL | 1.101 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Cy3 phosphoramidite | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| Cy3 tyramide | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| Cy3 aldehyde | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| Cy3B Tetrazine | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
| DBCO-Cy3 | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| Cy5 tetrazine | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
| XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| Cy7 tetrazine | 756 | 779 | 250000 | 0.3 | 0.05 | 0.036 |
| XFD350 tetrazine | 343 | 441 | 19000 | - | 0.25 | 0.19 |
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Citations
View all 1 citations: Citation Explorer
Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene--methyltetrazine reactions
Authors: Muraki, Michiro and Hirota, Kiyonori
Journal: BMC biotechnology (2017): 56
Authors: Muraki, Michiro and Hirota, Kiyonori
Journal: BMC biotechnology (2017): 56
References
View all 49 references: Citation Explorer
Beyond click chemistry - supramolecular interactions of 1,2,3-triazoles
Authors: Schulze B, Schubert US.
Journal: Chem Soc Rev (2014): 2522
Authors: Schulze B, Schubert US.
Journal: Chem Soc Rev (2014): 2522
Calixarene-based chemosensors by means of click chemistry
Authors: Song M, Sun Z, Han C, Tian D, Li H, Kim JS.
Journal: Chem Asian J (2014): 2344
Authors: Song M, Sun Z, Han C, Tian D, Li H, Kim JS.
Journal: Chem Asian J (2014): 2344
Use of click-chemistry in the development of peptidomimetic enzyme inhibitors
Authors: Fabbrizzi P, Menchi G, Guarna A, Trabocchi A.
Journal: Curr Med Chem (2014): 1467
Authors: Fabbrizzi P, Menchi G, Guarna A, Trabocchi A.
Journal: Curr Med Chem (2014): 1467
Applications of copper-catalyzed click chemistry in activity-based protein profiling
Authors: Martell J, Weerapana E.
Journal: Molecules (2014): 1378
Authors: Martell J, Weerapana E.
Journal: Molecules (2014): 1378
'Click chemistry' for diagnosis: a patent review on exploitation of its emerging trends
Authors: M, undefined and hare A, Banerjee P, Bhutkar S, Hirwani R.
Journal: Expert Opin Ther Pat (2014): 1287
Authors: M, undefined and hare A, Banerjee P, Bhutkar S, Hirwani R.
Journal: Expert Opin Ther Pat (2014): 1287
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