Calculator
DBCO-Cy3
This azadibenzocyclooctyne-cyanine dye derivative is a versatile labeling reagent for detection of azide containing molecules or compounds. Cyclooctynes are useful in strain-promoted copper-free azide-alkyne cycloaddition reactions. This dibenzocyclooctyne will react with azide-functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage. AAT Bioquest offer a broad range of dye azide compounds (such as coumarin azides, fluorescein azides, rhodamine azides and cyanine azides) for click reactions.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DBCO-Cy3 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 84.348 µL | 421.742 µL | 843.483 µL | 4.217 mL | 8.435 mL |
| 5 mM | 16.87 µL | 84.348 µL | 168.697 µL | 843.483 µL | 1.687 mL |
| 10 mM | 8.435 µL | 42.174 µL | 84.348 µL | 421.742 µL | 843.483 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Cy3 tetrazine | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| DBCO-Cy5 | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
| Cy3 phosphoramidite | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| Cy3 tyramide | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| Cy3 aldehyde | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| Cy3B DBCO | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
Citations
View all 2 citations: Citation Explorer
Imaging of antitubercular dimeric boronic acids at the mycobacterial cell surface by click-probe capture
Authors: Guy, Collette S and Tom{\'a}s, Ruben MF and Tang, Qiao and Gibson, Matthew I and Fullam, Elizabeth
Journal: Chemical Communications (2022): 9361--9364
Authors: Guy, Collette S and Tom{\'a}s, Ruben MF and Tang, Qiao and Gibson, Matthew I and Fullam, Elizabeth
Journal: Chemical Communications (2022): 9361--9364
Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene--methyltetrazine reactions
Authors: Muraki, Michiro and Hirota, Kiyonori
Journal: BMC biotechnology (2017): 56
Authors: Muraki, Michiro and Hirota, Kiyonori
Journal: BMC biotechnology (2017): 56
References
View all 49 references: Citation Explorer
Beyond click chemistry - supramolecular interactions of 1,2,3-triazoles
Authors: Schulze B, Schubert US.
Journal: Chem Soc Rev (2014): 2522
Authors: Schulze B, Schubert US.
Journal: Chem Soc Rev (2014): 2522
Calixarene-based chemosensors by means of click chemistry
Authors: Song M, Sun Z, Han C, Tian D, Li H, Kim JS.
Journal: Chem Asian J (2014): 2344
Authors: Song M, Sun Z, Han C, Tian D, Li H, Kim JS.
Journal: Chem Asian J (2014): 2344
Use of click-chemistry in the development of peptidomimetic enzyme inhibitors
Authors: Fabbrizzi P, Menchi G, Guarna A, Trabocchi A.
Journal: Curr Med Chem (2014): 1467
Authors: Fabbrizzi P, Menchi G, Guarna A, Trabocchi A.
Journal: Curr Med Chem (2014): 1467
Applications of copper-catalyzed click chemistry in activity-based protein profiling
Authors: Martell J, Weerapana E.
Journal: Molecules (2014): 1378
Authors: Martell J, Weerapana E.
Journal: Molecules (2014): 1378
'Click chemistry' for diagnosis: a patent review on exploitation of its emerging trends
Authors: M, undefined and hare A, Banerjee P, Bhutkar S, Hirwani R.
Journal: Expert Opin Ther Pat (2014): 1287
Authors: M, undefined and hare A, Banerjee P, Bhutkar S, Hirwani R.
Journal: Expert Opin Ther Pat (2014): 1287
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