Calculator
DBCO-Cy5
This azadibenzocyclooctyne-cyanine dye derivative is a versatile labeling reagent for detection of azide containing molecules or compounds. Cyclooctynes are useful in strain-promoted copper-free azide-alkyne cycloaddition reactions. This dibenzocyclooctyne will react with azide-functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage. AAT Bioquest offer a broad range of dye azide compounds (such as coumarin azides, fluorescein azides, rhodamine azides and cyanine azides) for click reactions.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DBCO-Cy5 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 82.535 µL | 412.677 µL | 825.355 µL | 4.127 mL | 8.254 mL |
| 5 mM | 16.507 µL | 82.535 µL | 165.071 µL | 825.355 µL | 1.651 mL |
| 10 mM | 8.254 µL | 41.268 µL | 82.535 µL | 412.677 µL | 825.355 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (482 nm) | Correction Factor (565 nm) |
| Cy5 tertrazine [Cy5 tertrazine] | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
| DBCO-Cy3 | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 | - | - |
| Cy5 phosphoramidite | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
| Cy5 tyramide | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
| Cy5 tetrazine | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
| Cy5 aldehyde | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
| Cy3B DBCO | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 | - | - |
Citations
View all 2 citations: Citation Explorer
Synthesis, characterization, and biological activity of spherical nucleic acid (SNA) constructs for cancer therapy and imaging
Authors: Calabrese, Colin
Journal: (2017)
Authors: Calabrese, Colin
Journal: (2017)
Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene--methyltetrazine reactions
Authors: Muraki, Michiro and Hirota, Kiyonori
Journal: BMC biotechnology (2017): 56
Authors: Muraki, Michiro and Hirota, Kiyonori
Journal: BMC biotechnology (2017): 56
References
View all 49 references: Citation Explorer
Beyond click chemistry - supramolecular interactions of 1,2,3-triazoles
Authors: Schulze B, Schubert US.
Journal: Chem Soc Rev (2014): 2522
Authors: Schulze B, Schubert US.
Journal: Chem Soc Rev (2014): 2522
Calixarene-based chemosensors by means of click chemistry
Authors: Song M, Sun Z, Han C, Tian D, Li H, Kim JS.
Journal: Chem Asian J (2014): 2344
Authors: Song M, Sun Z, Han C, Tian D, Li H, Kim JS.
Journal: Chem Asian J (2014): 2344
Use of click-chemistry in the development of peptidomimetic enzyme inhibitors
Authors: Fabbrizzi P, Menchi G, Guarna A, Trabocchi A.
Journal: Curr Med Chem (2014): 1467
Authors: Fabbrizzi P, Menchi G, Guarna A, Trabocchi A.
Journal: Curr Med Chem (2014): 1467
Applications of copper-catalyzed click chemistry in activity-based protein profiling
Authors: Martell J, Weerapana E.
Journal: Molecules (2014): 1378
Authors: Martell J, Weerapana E.
Journal: Molecules (2014): 1378
'Click chemistry' for diagnosis: a patent review on exploitation of its emerging trends
Authors: M, undefined and hare A, Banerjee P, Bhutkar S, Hirwani R.
Journal: Expert Opin Ther Pat (2014): 1287
Authors: M, undefined and hare A, Banerjee P, Bhutkar S, Hirwani R.
Journal: Expert Opin Ther Pat (2014): 1287
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