Cy5 tetrazine

Name: Cy5 tetrazine
Brand: AAT Bioquest
SKU: 902
Price: 227 USD
Availability: InStock

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This inverse electron demand cycloaddition reaction has gained popularity due to the potential for extremely fast cycloaddition kinetics with TCO as the dienophile. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this poweful click reaction. Cy5 tetrazine has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. It is widely used for labeling peptides, proteins and oligos etc.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cy5 tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	87.989 µL	439.947 µL	879.894 µL	4.399 mL	8.799 mL
5 mM	17.598 µL	87.989 µL	175.979 µL	879.894 µL	1.76 mL
10 mM	8.799 µL	43.995 µL	87.989 µL	439.947 µL	879.894 µL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Cy5 tertrazine [Cy5 tertrazine]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Cy5 phosphoramidite	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Cy5 tyramide	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Cy5 aldehyde	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Cy3 tetrazine	555	569	150000¹	0.15¹	0.07	0.073
DBCO-Cy5	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
XFD488 tetrazine Same Structure to Alexa Fluor™ 488 tetrazine	499	520	71000	0.92¹	0.30	0.11
Cy3B Tetrazine	560	571	120000¹	0.58¹	0.048	0.069
Cy7 tetrazine	756	779	250000	0.3	0.05	0.036
XFD350 tetrazine	343	441	19000	-	0.25	0.19
XFD405 Tetrazine	401	421	35,000	-	0.23	0.70
XFD430 Tetrazine	432	540	15,000	-	-	0.28
XFD514 Tetrazine	518	543	80000	-	0.31	0.18
XFD546 Tetrazine	561	572	112000	0.79¹	0.21	0.12
XFD532 Tetrazine	534	553	81000	0.61¹	0.24	0.09
XFD568 Tetrazine	579	603	91300	0.69¹	0.45	0.46
XFD594 Tetrazine	590	618	90000	0.66¹	0.43	0.56
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Page updated on November 21, 2024

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Catalog Number	902
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Physical properties

Molecular weight	1136.50
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.02
Correction Factor (280 nm)	0.03
Correction Factor (482 nm)	0.009
Correction Factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27¹, 0.4²