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Cy5.5 biotin conjugate

This bifunctional Cy5.5 biotin conjugate demonstrates excellent avidin binding and strong fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. Cy5.5 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and emission. Cy5.5 Biotin is a water-soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10.

Spectrum

References

View all 16 references: Citation Explorer
High-affinity peptide ligand LXY30 for targeting α3β1 integrin in non-small cell lung cancer.
Authors: Xiao, Wenwu and Ma, Weijie and Wei, Sixi and Li, Qianping and Liu, Ruiwu and Carney, Randy P and Yang, Kevin and Lee, Joyce and Nyugen, Alan and Yoneda, Ken Y and Lam, Kit S and Li, Tianhong
Journal: Journal of hematology & oncology (2019): 56
Carboxylesterase-Cleavable Biotinylated Nanoparticle for Tumor-Dual Targeted Imaging.
Authors: Chen, Peiyao and Kuang, Wen and Zheng, Zhen and Yang, Shuye and Liu, Yaling and Su, Lanhong and Zhao, Kui and Liang, Gaolin
Journal: Theranostics (2019): 7359-7369
Intracellular Proteolytic Disassembly of Self-Quenched Near-Infrared Nanoparticles Turning Fluorescence on for Tumor-Targeted Imaging.
Authors: Jiang, Jinhui and Zhao, Zhibin and Hai, Zijuan and Wang, Hongyong and Liang, Gaolin
Journal: Analytical chemistry (2017): 9625-9628
Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues.
Authors: Tulloch, Lindsay B and Menzies, Stefanie K and Fraser, Andrew L and Gould, Eoin R and King, Elizabeth F and Zacharova, Marija K and Florence, Gordon J and Smith, Terry K
Journal: PLoS neglected tropical diseases (2017): e0005886
SPECT/NIRF Dual Modality Imaging for Detection of Intraperitoneal Colon Tumor with an Avidin/Biotin Pretargeting System.
Authors: Dong, Chengyan and Yang, Sujuan and Shi, Jiyun and Zhao, Huiyun and Zhong, Lijun and Liu, Zhaofei and Jia, Bing and Wang, Fan
Journal: Scientific reports (2016): 18905
Page updated on November 21, 2024

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Catalog Number3105
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Physical properties

Molecular weight

1299.45

Solvent

Water

Spectral properties

Correction Factor (260 nm)

0.05

Correction Factor (280 nm)

0.101

Correction Factor (482 nm)

0.0017

Correction Factor (565 nm)

0.047

Correction Factor (650 nm)

0.454

Extinction coefficient (cm -1 M -1)

250000

Excitation (nm)

683

Emission (nm)

703

Quantum yield

0.27

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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