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Cy3 aldehyde
Cy3 aldehyde is a reactive fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated Cy3 dye can be easily detected by a common fluorescence instrument under TRITC or Cy3 channel.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Cy3 tetrazine | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| Cy3 phosphoramidite | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| Cy3 tyramide | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| DBCO-Cy3 | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| Fluorescein aldehyde [5-FAM aldehyde] | 493 | 517 | 83000 | - | 0.32 | 0.178 |
| Rhodamine aldehyde [5-TAMRA aldehyde] | 552 | 578 | 90000 | - | 0.32 | 0.178 |
| Cy5 aldehyde | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
| XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
References
View all 15 references: Citation Explorer
Resonance Energy Transfer-Based Nucleic Acid Hybridization Assays on Paper-Based Platforms Using Emissive Nanoparticles as Donors.
Authors: Doughan, Samer and Noor, M Omair and Han, Yi and Krull, Ulrich J
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 301-326
Authors: Doughan, Samer and Noor, M Omair and Han, Yi and Krull, Ulrich J
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 301-326
Development of a DNA microarray-based multiplex assay of avian influenza virus subtypes H5, H7, H9, N1, and N2.
Authors: Shi, L and Sun, J-S and Yang, Z-P and Bao, H-M and Jiang, Y-P and Xiong, Y-Z and Cao, D and Yu, X-W and Chen, H-L and Zheng, S-M and Wang, X-R
Journal: Acta virologica (2014): 14-9
Authors: Shi, L and Sun, J-S and Yang, Z-P and Bao, H-M and Jiang, Y-P and Xiong, Y-Z and Cao, D and Yu, X-W and Chen, H-L and Zheng, S-M and Wang, X-R
Journal: Acta virologica (2014): 14-9
The role of Src protein in the process formation of PC12 cells induced by the proteasome inhibitor MG-132.
Authors: Tarjányi, Oktávia and Berta, Gergely and Harci, Alexandra and Bacsa, Eszter B and Stark, Borbála and Pap, Marianna and Szeberényi, József and Sétáló, György
Journal: Neurochemistry international (2013): 413-22
Authors: Tarjányi, Oktávia and Berta, Gergely and Harci, Alexandra and Bacsa, Eszter B and Stark, Borbála and Pap, Marianna and Szeberényi, József and Sétáló, György
Journal: Neurochemistry international (2013): 413-22
Development and application of antibody microarray for lymphocystis disease virus detection in fish.
Authors: Sheng, Xiuzhen and Xu, Xiaoli and Zhan, Wenbin
Journal: Journal of virological methods (2013): 243-9
Authors: Sheng, Xiuzhen and Xu, Xiaoli and Zhan, Wenbin
Journal: Journal of virological methods (2013): 243-9
A multiplexed screening method for agonists and antagonists of the estrogen receptor protein.
Authors: Li, Zhonghui and Yan, Ming and Li, Zhoumin and Vuki, Maika and Wu, Dan and Liu, Fei and Zhong, Wenying and Zhang, Luyong and Xu, Danke
Journal: Analytical and bioanalytical chemistry (2012): 1373-84
Authors: Li, Zhonghui and Yan, Ming and Li, Zhoumin and Vuki, Maika and Wu, Dan and Liu, Fei and Zhong, Wenying and Zhang, Luyong and Xu, Danke
Journal: Analytical and bioanalytical chemistry (2012): 1373-84
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