Cy7 tetrazine

Cy7 tetrazine is an excellent building block that can be readily used to label TCO-modified biological molecules (e.g., peptides, proteins and oligos etc) for NIR fluorescence in vivo imaging and other fluorescence-based biological applications. The inverse electron demand Diels–Alder (IEDDA) between 1,2,4,5-tetrazine and strained alkene (such as trans-cyclooctene, TCO) is a well-established bioorthogonal reaction. It is generally considered to be the fastest click reaction. Since the first report on IEDDA reaction, several kinds of strained alkenes/alkynes and tetrazine analogs have been synthesized, and these functional group pairs have been applied to drug discovery, life science research and nanomaterials. Due to the extremely rapid reaction rate of IEDDA under mild conditions such as room temperature, neutral pH, and in aqueous media, this reaction has been a highly useful ligation approach for a variety of bioconjugations. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Cy7 tyramide	756	779	250000	0.3	0.05	0.036
Cy7 Styramide Superior Replacement for Cy7 tyramide	756	779	250000	0.3	0.05	0.036
Cy3 tetrazine	555	569	150000¹	0.15¹	0.07	0.073
Cy5 tetrazine	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
XFD488 tetrazine Same Structure to Alexa Fluor™ 488 tetrazine	499	520	71000	0.92¹	0.30	0.11
Cy3B Tetrazine	560	571	120000¹	0.58¹	0.048	0.069
PerCP-Cy7	757	783	350000	-	-	-
XFD350 tetrazine	343	441	19000	-	0.25	0.19
XFD405 Tetrazine	401	421	35,000	-	0.23	0.70
XFD430 Tetrazine	432	540	15,000	-	-	0.28
XFD514 Tetrazine	518	543	80000	-	0.31	0.18
XFD546 Tetrazine	561	572	112000	0.79¹	0.21	0.12
XFD532 Tetrazine	534	553	81000	0.61¹	0.24	0.09
XFD568 Tetrazine	579	603	91300	0.69¹	0.45	0.46
XFD594 Tetrazine	590	618	90000	0.66¹	0.43	0.56
XFD610 tetrazine	611	629	144,000	-	0.43	0.44
XFD633 Tetrazine	632	650	159,000	-	-	0.55
XFD660 Tetrazine	663	691	132000	-	0.00	0.10
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References

View all 3 references: Citation Explorer

Signal Amplification Pretargeted PET/Fluorescence Imaging Based on Human Serum Albumin-Encapsulated GdF3 Nanoparticles for Diagnosis of Ovarian Cancer.
Authors: Shi, Xudong and Gao, Kai and Zhang, Guoxin and Zhang, Wenlong and Yang, Xingjiu and Gao, Ran
Journal: ACS biomaterials science & engineering (2022): 4956-4964

Enabling In Vivo Photocatalytic Activation of Rapid Bioorthogonal Chemistry by Repurposing Silicon-Rhodamine Fluorophores as Cytocompatible Far-Red Photocatalysts.
Authors: Wang, Chuanqi and Zhang, He and Zhang, Tao and Zou, Xiaoyu and Wang, Hui and Rosenberger, Julia E and Vannam, Raghu and Trout, William S and Grimm, Jonathan B and Lavis, Luke D and Thorpe, Colin and Jia, Xinqiao and Li, Zibo and Fox, Joseph M
Journal: Journal of the American Chemical Society (2021): 10793-10803

NIR-Cyanine Dye Linker: a Promising Candidate for Isochronic Fluorescence Imaging in Molecular Cancer Diagnostics and Therapy Monitoring.
Authors: Komljenovic, Dorde and Wiessler, Manfred and Waldeck, Waldemar and Ehemann, Volker and Pipkorn, Ruediger and Schrenk, Hans-Hermann and Debus, Jürgen and Braun, Klaus
Journal: Theranostics (2016): 131-41

Page updated on December 17, 2024

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Catalog Number	905
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Physical properties

Molecular weight	1162.54
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.05
Correction Factor (280 nm)	0.036
Correction Factor (482 nm)	0.0005
Correction Factor (565 nm)	0.0193
Correction Factor (650 nm)	0.165
Extinction coefficient (cm ^-1 M ^-1)	250000
Excitation (nm)	756
Emission (nm)	779
Quantum yield	0.3