XFD514 Tetrazine

• Ex/Em: 518/543 nm
• Extinction coefficient: 80,000 cm^-1M^-1
• Reactive Group: Tetrazine
• Click Chemistry Conjugation: Provides rapid, copper-free labeling of TCO-modified biomolecules
• Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
• Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

XFD514, manufactured by AAT Bioquest, is a green-fluorescent dye structurally identical to Alexa Fluor™ 514 (ThermoFisher). It is characterized by high photostability and exceptional brightness, making it highly suitable for advanced imaging and flow cytometry applications. The dye is water-soluble and remains pH-insensitive within a broad range (pH 4–10), ensuring consistent fluorescence performance across diverse experimental conditions. XFD514 offers robust and reliable labeling, enabling precise and reproducible results in fluorescence-based assays. It also serves as an effective replacement for rhodamine 6G in applications requiring enhanced performance.

XFD514 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.