XFD514 amine

• Ex/Em: 518/543 nm
• Extinction coefficient: 80,000 cm^-1M^-1
• Reactive Group: Amine
• Versatile Conjugation: Efficiently labels carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
• Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
• Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

XFD514, manufactured by AAT Bioquest, is a bright green-fluorescent dye that is structurally identical to Alexa Fluor™ 514 (ThermoFisher). With optimal excitation between 480-535 nm, it is ideally compatible with the 488 nm line of the Argon-Ion laser and the 532 nm line of the frequency-doubled Nd:YAG laser. XFD514 demonstrates excellent water solubility and remains stable over a wide pH range (pH 4–10), providing consistent and robust fluorescence signals. These properties make it highly suitable for advanced imaging techniques such as fluorescence microscopy and flow cytometry. XFD514 is a suitable replacement for rhodamine 6G.

XFD514 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.