XFD514 azide

XFD514, manufactured by AAT Bioquest, is a bright green-fluorescent dye that is structurally identical to Alexa Fluor™ 514 (ThermoFisher). With optimal excitation between 480-535 nm, it is ideally compatible with the 488 nm line of the Argon-Ion laser and the 532 nm line of the frequency-doubled Nd:YAG laser. XFD514 demonstrates excellent water solubility and remains stable over a wide pH range (pH 4–10), providing consistent and robust fluorescence signals. These properties make it highly suitable for advanced imaging techniques such as fluorescence microscopy and flow cytometry. XFD514 is a suitable replacement for rhodamine 6G.

The azide derivative of XFD514 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.