XFD405 azide
XFD405, manufactured by AAT Bioquest, is a blue-fluorescent dye that is structurally identical to Alexa Fluor™ 405 (ThermoFisher). This dye is water-soluble and optimized for excitation by the 407 nm krypton laser line or the 408 nm violet laser diode, making it suitable for a range of fluorescence-based techniques. XFD405 is pH-insensitive across a wide range (pH 4 - 10) and exhibits minimal quenching when conjugated to proteins, ensuring consistent fluorescence signals in live-cell imaging. With an excitation maximum at 401 nm and emission at 422 nm, XFD405 is well-suited for multicolor flow cytometry and super-resolution microscopy (STORM), providing reliable performance in applications requiring distinct spectral separation and photostability.
The azide derivative of XFD405 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide* | 499 | 520 | 71000 | 0.11 |
XFD647 Azide | 650 | 671 | 270000 | 0.03 |
XFD350 azide | 343 | 441 | 19000 | 0.19 |
XFD405 Tetrazine | 401 | 421 | 35,000 | 0.70 |
XFD405 TCO | 401 | 421 | 35,000 | 0.70 |
XFD430 azide | 432 | 540 | 15,000 | 0.28 |
XFD514 azide | 518 | 543 | 80000 | 0.18 |
XFD546 azide | 561 | 572 | 112000 | 0.12 |
XFD532 azide | 534 | 553 | 81000 | 0.09 |
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