XFD405 acid

• Ex/Em: 401/421 nm
• Extinction coefficient: 35,000 cm^-1M^-1
• Consistent Fluorescence: pH-insensitive from pH 4 to 10 and minimal quenching when conjugated to proteins
• High Photostability: Ensures reliable performance for multicolor flow cytometry and advanced imaging applications like STORM

XFD405, manufactured by AAT Bioquest, is a blue-fluorescent dye that is structurally identical to Alexa Fluor™ 405 (ThermoFisher). This dye is water-soluble and optimized for excitation by the 407 nm krypton laser line or the 408 nm violet laser diode, making it suitable for a range of fluorescence-based techniques. XFD405 is pH-insensitive across a wide range (pH 4 - 10) and exhibits minimal quenching when conjugated to proteins, ensuring consistent fluorescence signals in live-cell imaging. With an excitation maximum at 401 nm and emission at 422 nm, XFD405 is well-suited for multicolor flow cytometry and super-resolution microscopy (STORM), providing reliable performance in applications requiring distinct spectral separation and photostability.

XFD405 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing XFD405 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.