XFD350 azide
XFD350, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor® 350 (ThermoFisher). This blue-fluorescent dye exhibits strong absorption and moderate photostability, with consistent fluorescence across a broad pH range (pH 4-11). XFD350 is effectively excited by the 350 nm line of a UV laser, and its moderate brightness and stability make it particularly advantageous for direct imaging of high-abundance targets in both microscopy and flow cytometry applications.
The azide derivative of XFD350 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide* | 499 | 520 | 71000 | 0.11 |
XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350* | 343 | 441 | 19000 | 0.19 |
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid* | 343 | 441 | 19000 | 0.19 |
XFD647 Azide | 650 | 671 | 270000 | 0.03 |
XFD350 tetrazine | 343 | 441 | 19000 | 0.19 |
XFD350 TCO | 343 | 441 | 19000 | 0.19 |
XFD405 azide | 401 | 421 | 35,000 | 0.70 |
XFD430 azide | 432 | 540 | 15,000 | 0.28 |
XFD514 azide | 518 | 543 | 80000 | 0.18 |
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