XFD610 azide

• Ex/Em: 611/629 nm
• Extinction coefficient: 144,000 cm^-1M^-1
• Reactive Group: Azide
• Click Chemistry Conjugation: Efficiently and rapidly labels terminal alkynes via CuAAC and copper-free SPAAC
• Advanced Dye Properties: Excellent water solubility and superior optical properties to Texas Red™; structural match to Alexa Fluor™ 610
• Robust Signal Performance: Maintains high intensity and pH independence (pH 4-10) with exceptional photostability

XFD610, manufactured by AAT Bioquest, is a red-fluorescent dye that is structurally identical to Alexa Fluor™ 610 (ThermoFisher). The dye exhibits high photostability and strong fluorescence, making it well-suited for advanced imaging applications and flow cytometry. Its water solubility and pH-independent fluorescence over a broad range (pH 4–10) ensure consistent performance under diverse experimental conditions. XFD610 enables reproducible labeling with high signal intensity, providing reliable results in fluorescence-based assays. Additionally, it serves as a robust alternative to Texas Red™, offering improved optical properties for experiments requiring enhanced fluorescence output.

The azide derivative of XFD610 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.