XFD610 tetrazine

• Ex/Em: 611/629 nm
• Extinction coefficient: 144,000 cm^-1M^-1
• Reactive Group: Tetrazine
• Click Chemistry Conjugation: Provides rapid, copper-free labeling of TCO-modified biomolecules
• Advanced Dye Properties: Excellent water solubility and superior optical properties to Texas Red™; structural match to Alexa Fluor™ 610
• Robust Signal Performance: Maintains high intensity and pH independence (pH 4-10) with exceptional photostability

XFD610, manufactured by AAT Bioquest, is a red-fluorescent dye that is structurally identical to Alexa Fluor™ 610 (ThermoFisher). The dye exhibits high photostability and strong fluorescence, making it well-suited for advanced imaging applications and flow cytometry. Its water solubility and pH-independent fluorescence over a broad range (pH 4–10) ensure consistent performance under diverse experimental conditions. XFD610 enables reproducible labeling with high signal intensity, providing reliable results in fluorescence-based assays. Additionally, it serves as a robust alternative to Texas Red™, offering improved optical properties for experiments requiring enhanced fluorescence output.

XFD610 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.